Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00001330' > 
  2. Construct 'CONS00000208' > 
  3. Construct 'CONS00001254' > 
  4. Construct 'CONS00001298' > 
  5. Construct 'CONS00001379'

Construct

ID

CONS00001379

Name

p.Cirobu.REG.KH2012.C1:9941324-9941700>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : CggatccTTACCCCACAGTGATATAACTTCTTTATTTG Reverse : CATGccatggCCCTCGCATTCCACATTCTCG Lowercase letters correspond to XbaI/NcoI restriction site.

This construct is testing Regulatory Region

REG00001359: Cirobu.REG.KH2012.C1:9941324-9941700|C6ST-like7

Sequence

Show sequence

Length: 377

TTACCCCACAGTGATATAACTTCTTTTTTGTTTATCTCTTTGAATAAGGTTAAGGTAAAT
GGTAGATTAATTAAAACAGCGAAGAGAAAGGAATTAGCAGTAAAACGACAGTCGTTAATG
CATTAATTTTACTCTTAGTTCTTTAAATAACATGATCTTCGCTATTGTTTTCCAAAAAAT
ATGTTAATGTAATATGGTAATTGTTTATTTCCGCTCTAGAGAATCCTAGATTGCAAGGCC
TTTTGTAAATATTTAACGTCAGTAAATACCGTCTGATTACTTCTCGCAGATACTTGTTAC
ATTCCTAAGAGTAAACATAGAGTTTTCGAGATAGGCATTATCACTGTTAATGAAAACGAG
AATGTGGAATGCGAGGG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data