- Construct 'CONS00001378'
Construct
ID
CONS00001378
Name
p.Cirobu.REG.KH2012.C12:712425-712503>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : AtctagaGAATTTACTCGATATTTGTATTGTTACGTC Reverse : TTccatggTCACATATATGCGTTGTGACGTCATATAC Lowercase letters correspond to XbaI/NcoI restriction site.
REG00001358: Cirobu.REG.KH2012.C12:712425-712503|CbpD,CbpE,CbpN
GAATTTACTCGATATTTGTATTGTTACGTCACTATTTGTTCTTGTTGTTTGTATATGACG
TCACAACGCATATATGTGA