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CONSTRUCT

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  1. Construct 'CONS00001378'

Construct

ID

CONS00001378

Name

p.Cirobu.REG.KH2012.C12:712425-712503>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : AtctagaGAATTTACTCGATATTTGTATTGTTACGTC Reverse : TTccatggTCACATATATGCGTTGTGACGTCATATAC Lowercase letters correspond to XbaI/NcoI restriction site.

This construct is testing Regulatory Region

REG00001358: Cirobu.REG.KH2012.C12:712425-712503|CbpD,CbpE,CbpN

Sequence

Show sequence

Length: 79

GAATTTACTCGATATTTGTATTGTTACGTCACTATTTGTTCTTGTTGTTTGTATATGACG
TCACAACGCATATATGTGA