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  1. Construct 'CONS00001374'

Construct

ID

CONS00001374

Name

p.Cirobu.REG.KH2012.C12:712628-713987>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives no LacZ expression in notochord. Sequence of the primers used : Forward : AtctagaAGAAATGTTTAATATTGCCCAAGTCCC Reverse : TggatccTCCGGACGACGCCATTCC Lowercase letters correspond to XbaI/BamHI restriction site.

This construct is testing Regulatory Region

REG00001354: Cirobu.REG.KH2012.C12:712628-713987|CbpD,CbpE,CbpN

Sequence

Show sequence

Length: 1,360

AGAAATGTTTAATATTGCCCAAGTCCCCTTGACGTCACAAATAATTAATTTTCTTCTCTA
TGTGACGTTGAGTCTGTTTGTTTACCTCAGATAGGTTCGAACCCGGTTAACCCTTAGCCT
TCAACCTCATTTACCAACACGTGACAGATAAATTAGTTTGTTGTCACCTGCACGGGTTTT
GAACCTTAAACCCCACGCCTCTTGCGATAGCATTCAAGCATCTGCGGACTCGAAATTCTA
GGCATCTCTTCAATGTTAAGAGTGACACTGACACGCTATGTTGTTATATAAACTAGGGAA
GTGTTTTGTATTTGCCGGGTAAGCGTGGAATGCGGCACACCCCGTAACCCCGAGCAAGCA
ACACGTATTTCCATCCATTCGTGACCAACCCTCCACACTTAACCATCGTATATGTTTGAA
CGTTTATCTTCACCCAAACCCAACTTACCTACACTGAGGTTTGTTTGATGTATTTGCTGG
CGTTTGCGCAATGCTACTACAGCATTATCTTATGTAGCAATGCTGATAGTTTCGTATAAC
TTATTATAATCGCTCCAAAAAGGTTGCAGCATTTCACACCATACTTTATGCCACACTGTA
ATAGTTTAATCGAATACAACTTAAATATGACTTTTGTTACTTACGAAGAAATGCTTGAAT
AGATCGTGACAACGCGAATCTGTATTATATTATCTCCATTCGTCGTTATAGCTACGGGGG
AATTTTTTCCCCCCCTTTTCGTTATTTTTTTTTTTTAAAAAAAAAAAAATTGACGAAAGA
GCGACAGGAAATAACGACAAACGACATTCTATTTTGGTGTCGCCTAAAAATAGCGTGTGT
GTAAAGAACGGCCTTTACCGAATATAAACCGCACATTTAGTCGCGTGTGTATTTTATAAA
ATATCCTGAATATTTTTGTGATGGGCTAAAAGTCACGTGCTTATATTCTATTGTATGTTG
TGCCACGTCACAATACAGTAAGTGATTTGTTATCGATGAGGGGTTGTGTGAATTCCGTGC
CACTCCCAACTTGGATGACAAGGGGATTAATCACGGAAAGCTGCAACTTCCACCTGGTAA
TCACGTTTTCACTACGTGCACATTCATTTATTCCACAACCAAAAAAAAAAATCAAAAAAG
AGAAAACAGACTTTTTATTACCCACAAAGTTACATATACGTTGTAATTCGTAAGCAGGCA
TGACGTGTACGAAACCGAACATTCGTGCTATAAAGACTGTCGTTGGCCTGCCACGCGAGG
ATAAAACAAGTTACAATCTTCCCTTCACCGCTAACGGTTAAATAGTGGTGTATGACGTCA
CATAGGACGTCCCGCGGGAGTTGGAATGGCGTCGTCCGGA

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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