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CONSTRUCT

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  1. Construct 'CONS00001406'
  2. Construct 'CONS00001373'

Construct

ID

CONS00001373

Name

p.Cirobu.REG.KH2012.C12:712425-714190>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord.

This construct is testing Regulatory Region

REG00001353: Cirobu.REG.KH2012.C12:712425-714190|CbpD,CbpE,CbpN

Sequence

Show sequence

Length: 1,766

TGCAACAATCATATCGTAATAATGACCGTTTGTTACGTCACAGACTGAAAGCGCTCGTTG
ATTCTATCGTCACAAAACTATCTTTTGAATAAACAAAATTCAGCGAATATTAACCCTTAA
TTAAACGACCATTATCAGCTTCCCATATTACGCATATTTCCGACCGTGGTTTGTTCAGCT
TACAAGTGTTGCTAAGTACCACTAGAAATGTTTAATATTGCCCAAGTCCCCTTGACGTCA
CAAATAATTAATTTTCTTCTCTATGTGACGTTGAGTCTGTTTGTTTACCTCAGATAGGTT
CGAACCCGGTTAACCCTTAGCCTTCAACCTCATTTACCAACACGTGACAGATAAATTAGT
TTGTTGTCACCTGCACGGGTTTTGAACCTTAAACCCCACGCCTCTTGCGATAGCATTCAA
GCATCTGCGGACTCGAAATTCTAGGCATCTCTTCAATGTTAAGAGTGACACTGACACGCT
ATGTTGTTATATAAACTAGGGAAGTGTTTTGTATTTGCCGGGTAAGCGTGGAATGCGGCA
CACCCCGTAACCCCGAGCAAGCAACACGTATTTCCATCCATTCGTGACCAACCCTCCACA
CTTAACCATCGTATATGTTTGAACGTTTATCTTCACCCAAACCCAACTTACCTACACTGA
GGTTTGTTTGATGTATTTGCTGGCGTTTGCGCAATGCTACTACAGCATTATCTTATGTAG
CAATGCTGATAGTTTCGTATAACTTATTATAATCGCTCCAAAAAGGTTGCAGCATTTCAC
ACCATACTTTATGCCACACTGTAATAGTTTAATCGAATACAACTTAAATATGACTTTTGT
TACTTACGAAGAAATGCTTGAATAGATCGTGACAACGCGAATCTGTATTATATTATCTCC
ATTCGTCGTTATAGCTACGGGGGAATTTTTTCCCCCCCTTTTCGTTATTTTTTTTTTTTA
AAAAAAAAAAAATTGACGAAAGAGCGACAGGAAATAACGACAAACGACATTCTATTTTGG
TGTCGCCTAAAAATAGCGTGTGTGTAAAGAACGGCCTTTACCGAATATAAACCGCACATT
TAGTCGCGTGTGTATTTTATAAAATATCCTGAATATTTTTGTGATGGGCTAAAAGTCACG
TGCTTATATTCTATTGTATGTTGTGCCACGTCACAATACAGTAAGTGATTTGTTATCGAT
GAGGGGTTGTGTGAATTCCGTGCCACTCCCAACTTGGATGACAAGGGGATTAATCACGGA
AAGCTGCAACTTCCACCTGGTAATCACGTTTTCACTACGTGCACATTCATTTATTCCACA
ACCAAAAAAAAAAATCAAAAAAGAGAAAACAGACTTTTTATTACCCACAAAGTTACATAT
ACGTTGTAATTCGTAAGCAGGCATGACGTGTACGAAACCGAACATTCGTGCTATAAAGAC
TGTCGTTGGCCTGCCACGCGAGGATAAAACAAGTTACAATCTTCCCTTCACCGCTAACGG
TTAAATAGTGGTGTATGACGTCACATAGGACGTCCCGCGGGAGTTGGAATGGCGTCGTCC
GGAAATTGATGCCAGAAGTTTTATATTCGCGTTGAAGACTTTCATCTTATCTGTGACGTC
ACCGCATTACCTTTATACCGGTGACGTCATGGTTAACTTATCCACACAACGGTTCAACCG
TGACGTCGAATTTACTCGATATTTGTATTGTTACGTCACTATTTGTTCTTGTTGTTTGTA
TATGACGTCACAACGCATATATGTGA