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CONSTRUCT

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  1. Construct 'CONS00001333'
  2. Construct 'CONS00001247'
  3. Construct 'CONS00001370'

Construct

ID

CONS00001370

Name

p.Cirobu.REG.KH2012.C5:4472021-4472163>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord, tail muscles, mesenchyme and head epidermis. Sequence of the primers used : Forward : AtctagaCTTAACCTAGATTGAATGCATTCCG Reverse : AggatccACAGCATAGTCACAACGGCTTTC Lowercase letters correspond to XbaI/BamHI restriction site.

This construct is testing Regulatory Region

REG00001350: Cirobu.REG.KH2012.C5:4472042-4472163|Rfx1/2/3

Sequence

Show sequence

Length: 122

CTTAACCTAGATTGAATGCATTCCGTCTCGAGTACAAATGCCTTTCTGATTTGTTGTCTT
GTTTGTTTTGCGTCTCTCTCGCTCTTGTTCAATATTTAGAAAGCCGTTGTGACTATGCTG
TG