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- Construct 'CONS00001368'
Construct
ID
CONS00001368
Name
p.Cirobu.REG.KH2012.C5:4471987-4472163>LacZ
Min Promoter
Reporter
Comment
Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord. Sequence of the primers used : Forward : AtctagaCCAGTAATGAGTTTCGTACAGAATAC Reverse : AggatccACAGCATAGTCACAACGGCTTTC Lowercase letters correspond to XbaI/BamHI restriction site.
Show sequence
Length: 177
CCAGTAATGAGTTTCGTACAGAATACGTTGCGCCGTGAATTGAAATGTGTGTTAACTTAA
CCTAGATTGAATGCATTCCGTCTCGAGTACAAATGCCTTTCTGATTTGTTGTCTTGTTTG
TTTTGCGTCTCTCTCGCTCTTGTTCAATATTTAGAAAGCCGTTGTGACTATGCTGTG