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  1. Construct 'CONS00001362'

Construct

ID

CONS00001362

Name

p.Cirobu.REG.KH2012.C5:4471738-4472163>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord, mesenchyme, tail muscle and head epidermis. Sequence of the primers used : Forward : TtctagaTGCCAGGTATGTCTAAATTCAACATG Reverse : AggatccACAGCATAGTCACAACGGCTTTC Lowercase letters correspond to XbaI/BamHI restriction site.

This construct is testing Regulatory Region

REG00001342: Cirobu.REG.KH2012.C5:4471738-4472163|Rfx1/2/3

Sequence

Show sequence

Length: 426

TGCCAGGTATGTCTAAATTCAACAGCTTCGGTAATGGCGCATTCTTTGTCATAATTATAC
GGAGGCAGGCTGGTGTACGAAGAAATTCCATTTCGACACGACATGTTGCGCTGTAGTGAC
TCAAGTTTCAAAGTTATGATAAGCTTTAAGAAAAGTGTAGACAGGCGTACTGCTTAACAA
AACATTTGCAGACTATTAAAAATTATTTAAAGTATTAATAAGCGCTACTTTAATATTTTT
GTATGTTTACCAGTAATGAGTTTCGTACAGAATACGTTGCGCCGTGAATTGAAATGTGTG
TTAACTTAACCTAGATTGAATGCATTCCGTCTCGAGTACAAATGCCTTTCTGATTTGTTG
TCTTGTTTGTTTTGCGTCTCTCTCGCTCTTGTTCAATATTTAGAAAGCCGTTGTGACTAT
GCTGTG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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