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- Construct 'CONS00000318' >
- Construct 'CONS00000432'
Construct
ID
CONS00000432
Name
Ci-Erfa 11091/11293 pFOG::LacZ
Min Promoter
Reporter
Comment
pSP1.72-B3-s6:562995-B4-bpFOG-B5-B1-NLS-LacZ-B2
Activity observed in the animal neural precursors (a6.5 and b6.5). Much more cleare in b6.5 blastomers. This activity recapitulates the endogenous expression of the target gene, Ci-Erfa
This is the cloned sequence. Note the attb3 (AAAGTAGGCT) and attb4 (ACCCAACTTT) sites on the start and the end of the sequence respectively
AAAGTAGGCTCCGCCTCTCCTGCTATTGTCTTGCGGAAATGGGGTCGAGGAAAAGGGTTA
GTCAGATACAAGGCCCGATCAGCGTCCGAGGAAATCAGGTCGTCTCGCGAAGCGCTCGCG
CGTAACAACAAAGGAGACGCTTTCCATCGTGCTCTTGTCCGCCGACTTCTCGCTATCACA
CCGTGGAGAGAACAAAAGAGTGAATACCCACGCACCCAACTTT