ID

CONS00000752

Name

pCi-Nut1 -123/94 ZicL1 mutated 1 pVenus::EGFP

Min Promoter

bpVenus

Reporter

GFP

Comment

Construct containing the region upstream of Ci-Nut from -123bp to 94bp and where the first ZicL binding site was mutated in three bases. This construct can t drive any expression (construct named ZicL1(m457) in the paper).

Primers used for the construct : ZicL1(m457)F 5-GTCGCAAGACATtaTtTGCGTGTATTGTTTTTTTGAAACCCCGTC-3 and ZicL1(m457)R 5-GACGGGGTTTCAAAAAAACAATACACGCAaAtaATGTCTTGCGAC-3