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CONSTRUCT

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  1. Clone 'AHC0AAA232YE14' > 
  2. Construct 'CONS00000243' > 
  3. Construct 'CONS00000451' > 
  4. Construct 'CONS00000095'

Construct

ID

CONS00000095

Name

pBra -483/17 first codons ZicLb1 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains the Ci-Bra cis regulatory region from -483bp upstream the TSS and the 17 first codons which is mutated in ZicLb (call ZicLb1) DNA regulatory motifs, B1: CACAGCTGG go to CAgAcgTcG. Mutation in ZicLb1 DNA Binding motif abolishes reporter expression. The nucleotide +1 taken in reference corresponds to the first codon.

This construct is testing Regulatory Region

REG00000102: Ci-Bra -483/17 first codons ZicLb1 mutated

Reference(s)

Show in situ data