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CONSTRUCT

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  1. EST 'AHC0AAA230YI11_RM1' > 
  2. Construct 'CONS00000473' > 
  3. Construct 'CONS00000294' > 
  4. Construct 'CONS00000357' > 
  5. Construct 'CONS00000107'

Construct

ID

CONS00000107

Name

pBra -300/17 first codons Su(H)1 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains the Ci-Bra cis regulatory region from -300bp to the first 17 codons in which a single nucleotide substitution was created in the Su(H)1 motif (TTCCCAC to TTgCCAC). This subtitution results in reduced binding of a GST/Ci-Su(H) fusion protein. The mutagenized transgene exhibits weak staining in the tail muscles and trunk mesenchyme, but is essentially inactive in the notochord. The nucleotide +1 taken in reference corresponds to the first codon.

This construct is testing Regulatory Region

REG00000114: Ci-Bra -300/17 first codons Su(H)1 mutated

Reference(s)

Show in situ data