- Clone 'cign050m19' >
- Construct 'CONS00000317' >
- Construct 'CONS00000366'
Construct
ID
CONS00000366
Name
pfog -314/1 GATA mutated::NLS-LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
this construct contains ci-fog regulatory region from -314pb to +1pb (construct named pfog-m314 in the paper) and has a point mutation in each of the two GATA binding sites. Mutation of both GATA sites abolished activity in the animal pole.
Show sequence
Length: 320
AGGCTGGAGAAGACCAACATAAAGTATTTCAAAATTCAGGAAACGGTCCAAGACCGAAGC
TCCAAAGCTCTTGTGTTCAGTTAAACTCTGATAGTGAATAAGCTTCGTGTATTGTACCGG
CCCATTGTCAATCATGCAAACTTGATATTATATTGACAAGAGAAGAAGGCAGTTTAAATT
AAAACTCTAAAGTAGAGAGACATTAATCTCAGCTGACAAGGCAGGTGGTCACAGTAAGTT
CATTTAAATAGTTGGCCAACAATAGCCTTTCCAAGAAAGTATTTTTGTTCCAGGTCTATA
CAAAAATAACACACATAACC