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  1. Construct 'CONS00000127'

Construct

ID

CONS00000127

Name

pTitf1 -553/39 M12 mutated::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

this construct contains a LacZ transgene with the region from -553bp of the 5prime flanking Cititf1 enhancer sequence which contains mutations by transversion of the nucleotides in position -464bp to -460bp (GCGCT to CCCCT)(construct M12 in the paper). This electroporation showed a decrease of the endodermal expression.

Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates of this region in the paper : -355bp/+238bp

This construct is testing Regulatory Region

REG00000134: Cititf1 -553/39 M12 mutated

Reference(s)





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