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- Construct 'CONS00000657'
Construct
ID
CONS00000657
Name
pCi-Pitx -2735/-2540 First Smad site mutated :CES2:LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
Minimal enhancer upstream of Pitx gene from -2735 to -2540, called D1bcde in the paper. The Pax binding site is absent. The first Smad regulatory motif was mutated (GCCG=>ACCG). The mutation (m2 in the paper) affects the expression in the neurohypophysis primordium, anterior epidermis, ventral anterior sensory vesicle, but in the mesenchyme it seems that expression is higher.
The sequence is based on the first codon on the KH transcript.
Show sequence
Length: 195
ACGGATTAAGGTAATATTTCTTTTTATGTTACCGCCCAGTCGGTTCCTGACAATACACTG
ACAGGTAGTGCGAATAAAAGGCGCTCGTGGATGTAAAAAAACACTAATAATCTTCAGTAA
TTTCCGTGCCTTTCCTATGGGCGAAAACTCCCGCCGTGTGAAACGACTTCAAAAGGCGGA
CAGTCGCCGCCAAGG