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CONSTRUCT

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  1. Construct 'CONS00000318' > 
  2. Construct 'CONS00000300' > 
  3. Construct 'CONS00000119' > 
  4. Construct 'CONS00000613' > 
  5. Construct 'CONS00000637'

Construct

ID

CONS00000637

Name

pCi-RhoD 565/989 FoxF3/4 mutated pFOG::LacZ

Min Promoter

bpFOG

Reporter

LacZ

Comment

the third and fourth FoxF site were mutated and RhoD expression is lost.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : +498/+922
The two FoxF sites mutated (TGTT->TGGT) were remplaced by "-". Transcription start site is based on the start of the first exon of the KH transcript model.

This construct is testing Regulatory Region

REG00000675: Ci-RhoD 565/989 FoxF3/4 mutated

Sequence

Show sequence

Length: 424

ACTAAATTTAATTAGCAACCCGCTGTTGTTCAAAGGTGTACACGCCCACGGTGTTTGAGA
ATTACAGCAGAGAGGTGGAATTAAATCGAAAGAAGGCGGTGCTAACATTGTGGGATACGG
CGGGTATATATTAGTACATACGTGTTTATGCAAGACATCGTAGGCTTGTTGACTTAGTTT
CGGTTAGCATGGAAGTGTAGTCTATGTTCTACGTGGGTTATGTTCTTGTCGAGGATCTGG
GATTAGGGCTAAATTCAGCCTATTATTACCACAACATTCAGACTGCTTTTGTAGGTCCAA
ATGTAACATATGACTAGTGTGTTCTGATGTG-------AATACAATAAACTAGCGCAAAC
-------CCCATTATGTTTAGTCATATAGGTCATATGAGAGTAAATGAAGTGTAAACCAA
ATGT

Reference(s)

Show in situ data