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CONSTRUCT

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  1. Construct 'CONS00000638'

Construct

ID

CONS00000638

Name

pCi-RhoD 565/989 FoxF2/3/4 mutated pFOG::LacZ

Min Promoter

bpFOG

Reporter

LacZ

Comment

the second, third, and fourth FoxF sites were mutated and these mutations cause a reduced RhoD expression in B7.5 lineage.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : +498/+922
The second, third, and fourth FoxF sites mutated were remplaced by "-".
Transcription start site is based on the start of the first exon of the KH transcript model.

This construct is testing Regulatory Region

REG00000676: Ci-RhoD 565/989 FoxF2/3/4 mutated

Sequence

Show sequence

Length: 424

ACTAAATTTAATTAGCAACCCGCTGTTGTTCAAAGGTGTACACGCCCACGGTGTTTGAGA
ATTACAGCAGAGAGGTGGAATTAAATCGAAAGAAGGCGGTGCTAACATTGTGGGATACGG
CGGGTATATATTAGTACATACG-------GCAAGACATCGTAGGCTTGTTGACTTAGTTT
CGGTTAGCATGGAAGTGTAGTCTATGTTCTACGTGGGTTATGTTCTTGTCGAGGATCTGG
GATTAGGGCTAAATTCAGCCTATTATTACCACAACATTCAGACTGCTTTTGTAGGTCCAA
ATGTAACATATGACTAGTGTGTTCTGATGTG-------AATACAATAAACTAGCGCAAAC
-------CCCATTATGTTTAGTCATATAGGTCATATGAGAGTAAATGAAGTGTAAACCAA
ATGT

Reference(s)