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CONSTRUCT

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  1. Construct 'CONS00000487' > 
  2. Construct 'CONS00000289' > 
  3. Construct 'CONS00000435' > 
  4. Construct 'CONS00000369' > 
  5. Construct 'CONS00000640'

Construct

ID

CONS00000640

Name

pCi-RhoD 565/989 Ets1/2-2 mutated pFOG::LacZ

Min Promoter

bpFOG

Reporter

LacZ

Comment

the second Ets1/2 site was mutated -> decreaseof RhoD expression.
Be careful, coordinates in the paper are different from ANISEED coordinates. We took the KH transcript model as reference.
Coordinates in the paper : +498/+922
The Ets1/2 site mutated (GGA->AGA) was remplaced by "-".
Transcription start site is based on the start of the first exon of the KH transcript model.

This construct is testing Regulatory Region

REG00000678: Ci-RhoD 565/989 Ets1/2-2 mutated

Sequence

Show sequence

Length: 424

ACTAAATTTAATTAGCAACCCGCTGTTGTTCAAAGGTGTACACGCCCACGGTGTTTGAGA
ATTACAGCAGAGAGGTGGAATTAAATCGAAAGAAGGCGGTGCTAACATTGTGGGATACGG
CGGGTATATATTAGTACATACGTGTTTATGCAAGACATCGTAGGCTTGTTGACTTAGTTT
CGGTTAGCATGGAAGTGTAGTCTATGTTCTACGTGGGTTATGTTCTTGTCG------TGG
GATTAGGGCTAAATTCAGCCTATTATTACCACAACATTCAGACTGCTTTTGTAGGTCCAA
ATGTAACATATGACTAGTGTGTTCTGATGTGTGTTTATAATACAATAAACTAGCGCAAAC
TGTTTGTCCCATTATGTTTAGTCATATAGGTCATATGAGAGTAAATGAAGTGTAAACCAA
ATGT

Reference(s)

Show in situ data