Aniseed - Construct

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Construct 'CONS00000669'

Construct

ID

CONS00000669

Name

pCi-Pitx -2751/-2696 repeated x2/25bp spacer:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)-25-(ab) in the paper. The region named D1ab in the paper was repeat once, and between the two fragments there is a spacer region of 25bp. This region can drive expression in the neurohypophysis primordium.
The spacer region added was symbolized by "N". The sequence is based on the first codon on the KH transcript.

This construct is testing Regulatory Region

REG00000700: Ci-Pitx -2751/-2696 repeated x2/25bp spacer

Sequence

Show sequence

Length: 135

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGNNNNN
NNNNNNNNNNNNNNNNNNNNAAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTT
TATGTTGCCGCCCAG

Reference(s)

Show in situ data