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CONSTRUCT

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  1. Construct 'CONS00000696'
  2. Construct 'CONS00000848'
  3. Construct 'CONS00000665'

Construct

ID

CONS00000665

Name

pCi-Pitx -2751/-2696 repeated x2 Second Pax site deleted:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2751 to -2696, called D1(ab)(ab-Pdel) in the paper. The region named D1ab in the paper was repeated 2 times and the Pax site in the repeated region was deleted. This region can t drive expression in the neurohypophysis primordium.
The deleted sequence was replaced by "-"

This construct is testing Regulatory Region

REG00000696: Ci-Pitx -2751/-2696 repeated x2 Second Pax site deleted

Sequence

Show sequence

Length: 110

AAACGCGACGACCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGAAA--
------CCTCCACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAG

Reference(s)