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CONSTRUCT

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  1. Construct 'CONS00000751'

Construct

ID

CONS00000751

Name

pCi-Nut1 -51/94 pVenus::EGFP

Min Promoter

bpVenus

Reporter

LacZ

Comment

Construct containing the region upstream of Ci-Nut1 from -51bp to 94bp and inserted into the promoter of Venus reporter gene.

This construct isnt able to drive expression in neural tube (construct named -47bp in the paper).

Primers used for the construct : Forward primer NutUPSU-47/SalI 5-GTCAGTCGACGTCGCTTTGTGAAAATCTGG-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.

This construct is testing Regulatory Region

REG00000788: Ci-Nut1 -51/94

Sequence

Show sequence

Length: 145

TTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCGTACCAGTTTTACAGT
TTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACACTATATCAACAACAT
GGAAATTGATTTTGGATTTGCAAGA

Reference(s)