- Construct 'CONS00000751'
Construct
ID
CONS00000751
Name
pCi-Nut1 -51/94 pVenus::EGFP
Min Promoter
Reporter
Comment
Construct containing the region upstream of Ci-Nut1 from -51bp to 94bp and inserted into the promoter of Venus reporter gene.
This construct isnt able to drive expression in neural tube (construct named -47bp in the paper).
Primers used for the construct : Forward primer NutUPSU-47/SalI 5-GTCAGTCGACGTCGCTTTGTGAAAATCTGG-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.
TTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCGTACCAGTTTTACAGT
TTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACACTATATCAACAACAT
GGAAATTGATTTTGGATTTGCAAGA