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- Construct 'CONS00000748'
Construct
ID
CONS00000748
Name
pCi-Nut1 -235/94 pVenus::EGFP
Min Promoter
Reporter
Comment
Construct containing the region upstream of Ci-Nut1 from -235bp to 94bp and inserted into the promoter region of Venus reporter gene (construct named -231bp in the paper) .
Primers used for the construct : Forward primer Nut1UPSU-231/SalI 5-TGCCGTCGACTTCGTGGAAATAGGTTCGC-3 and the reverse primer Nut1UPSL/BamHI 5-CGCGGATCCTCCAAAATCAATTTCCATG-3.
Show sequence
Length: 329
CGTTTTTGTATTCGTGGAAATAGGTTCGCATTTTTTATTAAGAAAGAGTTTGGTTTTCGA
GGTCACTGAATTGCAATTAGAAGGCATTTAATAGTAGCACTAGGACACTCTATTCACTGC
GTTGTCTAAATCCATGCGAAACAACAAAATAAGTCGCAAGACATGCTGTGCGTGTATTGT
TTTTTTGAAACCCCGTCGCTTTGTGAAAATCTGGTTGATTATTTTTTCGTACCAGTTTTA
CAGTTTAAATACGACTGTGCTTCAGTTTTTGTTAGTATTGAGTTGTACACTATATCAACA
ACATGGAAATTGATTTTGGATTTGCAAGA