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CONSTRUCT

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  1. Construct 'CONS00000661'
  2. Construct 'CONS00000655'

Construct

ID

CONS00000655

Name

pCi-Pitx -2735/-2540 Second Otx site mutated:CES2:LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Minimal enhancer upstream of Pitx gene from -2735 to -2540, called D1bcde in the paper. The Pax binding site is absent. The second Otx motif was mutated (TAATC => TCATC). The mutation (m5/6 in the paper) affects the expression in the neurohypophysis primordium.

KH transcript model was taken as reference for the region s coordinates.
The sequence is based on the first codon on the KH transcript.

This construct is testing Regulatory Region

REG00000686: Ci-Pitx -2735/-2540 Second Otx site mutated

Sequence

Show sequence

Length: 195

ACGGATTAAGGTAATATTTCTTTTTATGTTGCCGCCCAGTCGGTTCCTGACAATACACTG
ACAGGTAGTGCGAATAAAAGGCGCTCGTGGATGTAAAAAAACACTAATAATCTTCAGTAA
TTTCCGTGCCTTTCCTATGGGCGAAAACTCCCGCCGTGTGAAACGACTTCAAAAGGCGGA
CAGTCGCCGCCAAGG

Reference(s)