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CONSTRUCT

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  1. Construct 'CONS00000701'
  2. Construct 'CONS00000258'
  3. Construct 'CONS00000697'

Construct

ID

CONS00000697

Name

pCi-Hox1 -2488/502 Intron 2 added delta 821bp::LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

Region upstream of Ci-Hox1 from -2488 to +502bp leading expression in epidermis, posterior trunk, and anterior tail. This region was named Ci-Hox(deltaBsSp) in the paper. In this construct, a part (1.9kb) of the second intron of Ci-Hox gene was added between the nucleotid -972 and -878 (the region between was deleted) in the regulatory region. There is an other modification : 821bp were deleted. The two conserved sequence elements between Ciona species and the RARE regulatory motif were absent, due to the 821bp deletion.

This construct is testing Regulatory Region

REG00000731: Ci-Hox1 -2488/502 Intron 2 added delta 821bp

Reference(s)