Login
Help

CONSTRUCT

Submit your Data

  1. Construct 'CONS00001627' > 
  2. Construct 'CONS00001587' > 
  3. Construct 'CONS00001651' > 
  4. Clone 'AHC0AAA232YE14' > 
  5. Construct 'CONS00001133'

Construct

ID

CONS00001133

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4204263.Deleted_4204077-4203951:prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2.3kpb region located upstream the start codon of cdc25 (KHC5.12) missing the cis-regulatory modules CRM1 and CRM2 (as defined by Ogura and Sasakura 2016), cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede. This construct drives no expression in the epidermis at gastrula stage.

This construct is testing Regulatory Region

REG00001159: Cirobu.REG.KH2012.C5:4201966-4204263.Deleted_4204077-4203951

Sequence

Show sequence

Length: 2,172

ATTCCGACGTGCTGTAACGGTCTTACCGCCCTTTGGTTAGCGCTGATGTTAAGTAGGTGG
CGTTGGCAGTTGGGCGACATATGTTTTACTGCAGTGAGAGTCTTAAAAAAATATCAAGTT
TTCTTTAAGTGGCAGAAATCTGTCGCCGTGGTTCTGCTCTTGTATTTTACTGTTCAGCAT
ATACAATGACGCTAGAATAGTGCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCG
CTACTTATCAGTGGACGACCAAAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGAC
TGCACCAGCACTCTTAAAATAGCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTG
CACATGCAGCGCACAACCAGCTGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGC
ATTATTAGCAATATTCCATGTAATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATT
CGTTAATTAAAACTAATCTAGTATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACT
AGTTGTGTGTTTTTCGTGCAACGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTC
AATATATATGTGGTGCTTCTCGTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTA
GAACCGACTCGCTGCCTAATCCGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAAT
CCCACCCGTTAATCCACTTGCGTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAAC
CGAGACATCCTGACTGCATCCAGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGAT
TTACTTGCCAGCAGCAAACTCGCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGA
CTCACCGCCAAAGGAAAAAGGTTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTA
GTCACTCGCACGCTGTGTCCCAGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTA
CATGAGCTGCCTTCAGAATATCAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCT
CATAGCTCTTGGGCCTTTTTTCTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCA
GTTTGAGCGCGTATTAAGCAGCGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAG
CATAAGAGTCAAACAGTATTCACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTA
AAACTTTATCATTACTGTACAACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCAT
TGCAGACCACATACTACGCATTGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATG
TATATAGGACAAGTGGCCAGTATCGACAGGCGGATGTATTCACATCTAAAGCACATAACT
GTAGCGTGAGGCTACCACGTTATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTG
CGTATGTATAGTAGTTTGTAGTGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAG
AGAAAGAGACTATGCTATTACATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCAC
AATAACTAGAATCTAAGAGTCGTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTG
GATATGTCATTTTATTGTGTTGATTAAGAGAGTACCATGCAGCATACATACATACGTTGT
ATTGTGTATTATATTGAGTGAGTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCC
TATACCTCGAGCGTAACATATGGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCG
ACGCAAGCGTTATAAAGTTGGATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGT
ATTGCCGATGGAATTGGGTCATATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGA
TATCTCGTGGATTAGCAGAGTATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCA
GAATTTCAGGTTATTTGACATCCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTG
AGAGCTTACTCTGTTGTAAGCTAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCT
GTCTTTAATATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

Show in situ data