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CONSTRUCT

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  1. Construct 'CONS00001074' > 
  2. Construct 'CONS00001072' > 
  3. Construct 'CONS00001076' > 
  4. Construct 'CONS00001070' > 
  5. Construct 'CONS00001075'

Construct

ID

CONS00001075

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4203951:prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede. At the gastrula stage, it does not drive expression in epidermis.

This construct is testing Regulatory Region

REG00001107: Cirobu.REG.KH2012.C5:4201966-4203951

Sequence

Show sequence

Length: 1,986

TGACGCTAGAATAGTGCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCGCTACTT
ATCAGTGGACGACCAAAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGACTGCACC
AGCACTCTTAAAATAGCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTGCACATG
CAGCGCACAACCAGCTGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGCATTATT
AGCAATATTCCATGTAATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATTCGTTAA
TTAAAACTAATCTAGTATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACTAGTTGT
GTGTTTTTCGTGCAACGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTCAATATA
TATGTGGTGCTTCTCGTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTAGAACCG
ACTCGCTGCCTAATCCGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAATCCCACC
CGTTAATCCACTTGCGTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAACCGAGAC
ATCCTGACTGCATCCAGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGATTTACTT
GCCAGCAGCAAACTCGCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGACTCACC
GCCAAAGGAAAAAGGTTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTAGTCACT
CGCACGCTGTGTCCCAGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTACATGAG
CTGCCTTCAGAATATCAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCTCATAGC
TCTTGGGCCTTTTTTCTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCAGTTTGA
GCGCGTATTAAGCAGCGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAGCATAAG
AGTCAAACAGTATTCACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTAAAACTT
TATCATTACTGTACAACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCATTGCAGA
CCACATACTACGCATTGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATGTATATA
GGACAAGTGGCCAGTATCGACAGGCGGATGTATTCACATCTAAAGCACATAACTGTAGCG
TGAGGCTACCACGTTATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTGCGTATG
TATAGTAGTTTGTAGTGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAGAGAAAG
AGACTATGCTATTACATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCACAATAAC
TAGAATCTAAGAGTCGTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTGGATATG
TCATTTTATTGTGTTGATTAAGAGAGTACCATGCAGCATACATACATACGTTGTATTGTG
TATTATATTGAGTGAGTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCCTATACC
TCGAGCGTAACATATGGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCGACGCAA
GCGTTATAAAGTTGGATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGTATTGCC
GATGGAATTGGGTCATATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGATATCTC
GTGGATTAGCAGAGTATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCAGAATTT
CAGGTTATTTGACATCCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTGAGAGCT
TACTCTGTTGTAAGCTAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCTGTCTTT
AATATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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