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CONSTRUCT

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  1. Construct 'CONS00000253' > 
  2. Construct 'CONS00001088' > 
  3. Construct 'CONS00001203' > 
  4. Construct 'CONS00001175' > 
  5. Construct 'CONS00001136'

Construct

ID

CONS00001136

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA_AP2-1 ,2deletion):prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), where both GATA binding sites of the CRM1 were mutated (as defined by Ogura and Sasakura 2016) and both AP-2 binding sites of CRM2 were deleted, cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede.

This construct is testing Regulatory Region

REG00001162: Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA_AP2-1,2deletion

Sequence

Show sequence

Length: 2,094

TGCCTGTATAACAATGTGCCGTCACAGAAGAAAACAATGGACTTTTGAAGGTGAAAGGAG
AGGCAATTCGTTGAACGGTCCTATTAGAACGAATCCAGCTCTTATTTATGACGCTAGAAT
AGTGCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCGCTACTTATCAGTGGACGA
CCAAAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGACTGCACCAGCACTCTTAAA
ATAGCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTGCACATGCAGCGCACAACC
AGCTGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGCATTATTAGCAATATTCCA
TGTAATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATTCGTTAATTAAAACTAATC
TAGTATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACTAGTTGTGTGTTTTTCGTG
CAACGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTCAATATATATGTGGTGCTT
CTCGTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTAGAACCGACTCGCTGCCTA
ATCCGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAATCCCACCCGTTAATCCACT
TGCGTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAACCGAGACATCCTGACTGCA
TCCAGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGATTTACTTGCCAGCAGCAAA
CTCGCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGACTCACCGCCAAAGGAAAA
AGGTTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTAGTCACTCGCACGCTGTGT
CCCAGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTACATGAGCTGCCTTCAGAA
TATCAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCTCATAGCTCTTGGGCCTTT
TTTCTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCAGTTTGAGCGCGTATTAAG
CAGCGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAGCATAAGAGTCAAACAGTA
TTCACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTAAAACTTTATCATTACTGT
ACAACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCATTGCAGACCACATACTACG
CATTGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATGTATATAGGACAAGTGGCC
AGTATCGACAGGCGGATGTATTCACATCTAAAGCACATAACTGTAGCGTGAGGCTACCAC
GTTATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTGCGTATGTATAGTAGTTTG
TAGTGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAGAGAAAGAGACTATGCTAT
TACATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCACAATAACTAGAATCTAAGA
GTCGTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTGGATATGTCATTTTATTGT
GTTGATTAAGAGAGTACCATGCAGCATACATACATACGTTGTATTGTGTATTATATTGAG
TGAGTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCCTATACCTCGAGCGTAACA
TATGGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCGACGCAAGCGTTATAAAGT
TGGATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGTATTGCCGATGGAATTGGG
TCATATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGATATCTCGTGGATTAGCAG
AGTATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCAGAATTTCAGGTTATTTGA
CATCCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTGAGAGCTTACTCTGTTGTA
AGCTAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCTGTCTTTAATATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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