Aniseed - Construct

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Construct 'CONS00001224'

Construct

ID

CONS00001224

Name

p.Cirobu.REG.KH2012.C6:403105-403359>LacZ

Min Promoter

Endogenous Promoter

Reporter

LacZ

Comment

This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to L construct in Pezzotti et al. 2014).

This construct is testing Regulatory Region

REG00001222: Cirobu.REG.KH2012.C6:403105-403359|Onecut_HNF6

Sequence

Show sequence

Length: 255

GCATAGACGTTATTCTAAACCAGCATTTAAGGATTTCTCAGCGGGAATCTTTGTAAAATT
TTGACGAAAATTTCAAATTTTTTAAATCTTTTTGTGAAATAACTGTAAAGCAGAAAATAA
AAGTTCAATTTTATTAAAGCTAAAGTCATTTTAACCAAATCAGTATTCGTTCCACAGTTC
CATATATACATTAACCTAACTAGGTACTTAATTGTGATTTCTCAATGAGTTATTCAATGA
TGCTTCAGATACTCT

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

Show in situ data