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CONSTRUCT

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  1. Construct 'CONS00001137' > 
  2. Construct 'CONS00001071' > 
  3. Construct 'CONS00001074'

Construct

ID

CONS00001074

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4203978(µGATA BS12):prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), where both GATA binding sites of the CRM1 were mutated (as defined by Ogura and Sasakura 2016), cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede. This construct directs expression in neural ectoderm and muscle at the gastrula stage, but the epidermal expression is lost.

This construct is testing Regulatory Region

REG00001106: Cirobu.REG.KH2012.C5:4201966-4203978|cdc25_µGATA BS12

Sequence

Show sequence

Length: 2,013

TATTAGAACGAATCCAGCTCTTATTTATGACGCTAGAATAGTGCGTGCCTCGGGGCCCGA
ACCGCGACGCTTCGGCCTTCGCTACTTATCAGTGGACGACCAAAACACACTGAACCTGTA
GTAAAGCGAGAAAAAGGCGACTGCACCAGCACTCTTAAAATAGCAGCTGCGGTTCGATGC
AGGGCACCATTCATTCAACTGCACATGCAGCGCACAACCAGCTGAGTTCAATTTGTTAGT
AGATCGATTCGGTATACATGCATTATTAGCAATATTCCATGTAATTGGAGAATATGCAAC
AGTGCAGGATAGTGACCGATTCGTTAATTAAAACTAATCTAGTATTCACTCATGTTCTGC
CTGCATAGTTAAAGGAAGACTAGTTGTGTGTTTTTCGTGCAACGTTAGTAGGACAGTAGT
AGTAAGCAAAAATACGTCGTCAATATATATGTGGTGCTTCTCGTAAATATCTCCGCGGAG
CAAACTTTTCTATAAAAATTAGAACCGACTCGCTGCCTAATCCGTAACTAGTTCCCAACT
TCGGATATATTAAGCAAGAATCCCACCCGTTAATCCACTTGCGTGCATCCATAGTGCCCG
CATAGCGTACAAACAGCCAACCGAGACATCCTGACTGCATCCAGCCGGGCCAAGGTGCGC
TAGTATTGTTGGTCGTAGGATTTACTTGCCAGCAGCAAACTCGCTACCGAGCGAGAGCCC
AGCGGTATGTTTCCTTTTCGACTCACCGCCAAAGGAAAAAGGTTAATCTCTCGCTTGGTA
ATCGCGTCACAGGTAAAGGTAGTCACTCGCACGCTGTGTCCCAGCTTGGCCGGATTGTAA
GATGCTTCGTAAACCAGTTTACATGAGCTGCCTTCAGAATATCAATTAGCGTCCAAACCG
GACAATGCAAGGGAATTAGCTCATAGCTCTTGGGCCTTTTTTCTCAACTTCCTAATCGTA
GACATCTTGCCTGGGATTTCAGTTTGAGCGCGTATTAAGCAGCGAGACAGACTTCGGCTC
TTATATATTACTAGACAGAAGCATAAGAGTCAAACAGTATTCACCTATTCAGCTACCTCT
AGGTAGTTACTGGTCTACGTAAAACTTTATCATTACTGTACAACAATGTAGTACCGTTCC
AGTATACCCAGAGCAGCGCATTGCAGACCACATACTACGCATTGTTATTGTTTACTTTGT
GCCGATAAGCCGTATCCAATGTATATAGGACAAGTGGCCAGTATCGACAGGCGGATGTAT
TCACATCTAAAGCACATAACTGTAGCGTGAGGCTACCACGTTATATAGCGACTATAGCGT
ATTGGTAACTGAAAGAAATTGCGTATGTATAGTAGTTTGTAGTGCGTTAAAACTAAAGTG
GAATGCTCCTCGAGCACTGAGAGAAAGAGACTATGCTATTACATGGTTGTTTAACTGACG
ATACTAGACGGTTCATTGCACAATAACTAGAATCTAAGAGTCGTATACTAGTTAGCTGGC
TATAGTATTATTGGGGTTTTGGATATGTCATTTTATTGTGTTGATTAAGAGAGTACCATG
CAGCATACATACATACGTTGTATTGTGTATTATATTGAGTGAGTAGTATTAGAATGTCGG
GTATATACTCAAAAGGCCTCCTATACCTCGAGCGTAACATATGGTGTGGCCATTGGAGCT
TATTGCAACAGTTGTTCTGCGACGCAAGCGTTATAAAGTTGGATTATGACTTAGTAAAGA
CCGCAATAACATAGTGGTCGTATTGCCGATGGAATTGGGTCATATCGGCGATGTAACTTA
TGGGCGTTTGTCATAAGCCGATATCTCGTGGATTAGCAGAGTATCTTCGCCGATCGAGTA
AGCAAACTGTTTTCTTTTGCAGAATTTCAGGTTATTTGACATCCGAAGTAGCTAATTGCG
TTTTAGACATACGGTATCTTGAGAGCTTACTCTGTTGTAAGCTAGCGGGTGTTATACACT
TGTGGTCAATCTTCAGCACCTGTCTTTAATATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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