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CONSTRUCT

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  1. Construct 'CONS00001490' > 
  2. Construct 'CONS00000261' > 
  3. Construct 'CONS00000119' > 
  4. Construct 'CONS00001226' > 
  5. Construct 'CONS00001219'

Construct

ID

CONS00001219

Name

p.Cirobu.REG.KH2012.C6:403105-403572>LacZ

Min Promoter

Cirobu.REG.KH2012.C6:403105-403572|Onecut_HNF6

Reporter

LacZ

Comment

This cis-regulatory sequence of Onecut/HNF6 drives LacZ activity in the ectopic mesenchyme in Ciona intestinalis tailbuds and larvae.
A human β-globin basal promoter is localized between the regulatory sequence and the LacZ reporter gene. (It corresponds to C construct in Pezzotti et al. 2014)

This construct is testing Regulatory Region

:

Sequence

Show sequence

Length: 468

GCATAGACGTTATTCTAAACCAGCATTTAAGGATTTCTCAGCGGGAATCTTTGTAAAATT
TTGACGAAAATTTCAAATTTTTTAAATCTTTTTGTGAAATAACTGTAAAGCAGAAAATAA
AAGTTCAATTTTATTAAAGCTAAAGTCATTTTAACCAAATCAGTATTCGTTCCACAGTTC
CATATATACATTAACCTAACTAGGTACTTAATTGTGATTTCTCAATGAGTTATTCAATGA
TGCTTCAGATACTCTGCTCATCATTAGAACTTGAAATTAAAACTAACTTGTTTTTCAACA
TCGTCAAAACTTAGACGCATAAAATGTACGCGCATATAATAAGTCAAAAATGTTACAAAG
TTGCCAAATATTTAAAAATTGGAATATTATGTTTAACTTAAATGATACCATTTTTTGATT
TATGATATATGGTTTAGAAATTGACAACGAACTCCGAAGAATTCAACG

Reference(s)

Pezzotti et al. (2014). Auto and cross regulatory elements control Onecut expression in the ascidian nervous system. Dev. Biol. 390(2):273-87

Show in situ data