Aniseed - Construct

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Construct 'CONS00001265'

Construct

ID

CONS00001265

Name

p.Cirobu.REG.KH2012.C7:40900-40993_Fox2/AP1mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested fragment has no enhancer activity in the notochord and is weakly active in mesenchyme and central nervous system.

This construct is testing Regulatory Region

REG00001262: Cirobu.REG.KH2012.C7:40900-40993_Fox2/AP1mut|Noe2; Olm2A/B

Sequence

Show sequence

Length: 94

GAATGTTACGCGTTAGAAAGTCGAAACAGGGCGAACAAATACTCTTCGGTTCGGCACTGA
TGCGAAATTAGAACCCAAGTCTCGCTCTTTTCGC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data