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CONSTRUCT

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  1. Construct 'CONS00001137' > 
  2. Construct 'CONS00001071' > 
  3. Construct 'CONS00001074' > 
  4. Construct 'CONS00001072' > 
  5. Construct 'CONS00001076'

Construct

ID

CONS00001076

Name

pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA):prCirobu.bpForkhead>Kaede

Min Promoter

pfkh

Reporter

pSP-Kaede

Comment

This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), where both GATA binding sites of the CRM1 were mutated (as defined by Ogura and Sasakura 2016), cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede. This construct directs epidermal expression dunring gastrula

This construct is testing Regulatory Region

REG00001108: Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA

Sequence

Show sequence

Length: 2,112

TGCCTGTATAACAATGGCCCACCTGTGCCGTCACAGAAGAAAACAATGGACTTTTGAGCC
GTGGACAGGTGAAAGGAGAGGCAATTCGTTGAACGGTCCTATTAGAACGAATCCAGCTCT
TATTTATGACGCTAGAATAGTGCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCG
CTACTTATCAGTGGACGACCAAAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGAC
TGCACCAGCACTCTTAAAATAGCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTG
CACATGCAGCGCACAACCAGCTGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGC
ATTATTAGCAATATTCCATGTAATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATT
CGTTAATTAAAACTAATCTAGTATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACT
AGTTGTGTGTTTTTCGTGCAACGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTC
AATATATATGTGGTGCTTCTCGTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTA
GAACCGACTCGCTGCCTAATCCGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAAT
CCCACCCGTTAATCCACTTGCGTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAAC
CGAGACATCCTGACTGCATCCAGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGAT
TTACTTGCCAGCAGCAAACTCGCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGA
CTCACCGCCAAAGGAAAAAGGTTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTA
GTCACTCGCACGCTGTGTCCCAGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTA
CATGAGCTGCCTTCAGAATATCAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCT
CATAGCTCTTGGGCCTTTTTTCTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCA
GTTTGAGCGCGTATTAAGCAGCGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAG
CATAAGAGTCAAACAGTATTCACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTA
AAACTTTATCATTACTGTACAACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCAT
TGCAGACCACATACTACGCATTGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATG
TATATAGGACAAGTGGCCAGTATCGACAGGCGGATGTATTCACATCTAAAGCACATAACT
GTAGCGTGAGGCTACCACGTTATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTG
CGTATGTATAGTAGTTTGTAGTGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAG
AGAAAGAGACTATGCTATTACATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCAC
AATAACTAGAATCTAAGAGTCGTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTG
GATATGTCATTTTATTGTGTTGATTAAGAGAGTACCATGCAGCATACATACATACGTTGT
ATTGTGTATTATATTGAGTGAGTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCC
TATACCTCGAGCGTAACATATGGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCG
ACGCAAGCGTTATAAAGTTGGATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGT
ATTGCCGATGGAATTGGGTCATATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGA
TATCTCGTGGATTAGCAGAGTATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCA
GAATTTCAGGTTATTTGACATCCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTG
AGAGCTTACTCTGTTGTAAGCTAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCT
GTCTTTAATATG

Reference(s)

Ogura et al. (2016). Developmental Control of Cell-Cycle Compensation Provides a Switch for Patterned Mitosis at the Onset of Chordate Neurulation. Dev. Cell 37(2):148-61

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