- Construct 'CONS00001137'
Construct
ID
CONS00001137
Name
pSP.Cirobu.REG.KH2012.C5:4201966-4204077(µGATA_Sox deletion):prCirobu.bpForkhead>Kaede
Min Promoter
Reporter
Comment
This reporter construct contains the 2kpb region located upstream the start codon of cdc25 (KHC5.12), where both GATA binding sites of the CRM1 were mutated (as defined by Ogura and Sasakura 2016)and the three Sox binding sites of CRM2 were deleted, cloned upstream the basal promoter of Forkhead and the reporter gene pSP Kaede. This construct drives no expression in the epidermis at gastrula stage.
REG00001163: Cirobu.REG.KH2012.C5:4201966-4204077/cdc25_µGATA_Sox deletion
Show sequence
Length: 2,091
TGCCTGTATGCCCACCTGTGCCGTCACAGAAGAAGACTTTTGAGCCGTGGACAGGTGAAA
GGAGAGGCAATTCGGTCCTATTAGAACGAATCCAGCTCTTATTTATGACGCTAGAATAGT
GCGTGCCTCGGGGCCCGAACCGCGACGCTTCGGCCTTCGCTACTTATCAGTGGACGACCA
AAACACACTGAACCTGTAGTAAAGCGAGAAAAAGGCGACTGCACCAGCACTCTTAAAATA
GCAGCTGCGGTTCGATGCAGGGCACCATTCATTCAACTGCACATGCAGCGCACAACCAGC
TGAGTTCAATTTGTTAGTAGATCGATTCGGTATACATGCATTATTAGCAATATTCCATGT
AATTGGAGAATATGCAACAGTGCAGGATAGTGACCGATTCGTTAATTAAAACTAATCTAG
TATTCACTCATGTTCTGCCTGCATAGTTAAAGGAAGACTAGTTGTGTGTTTTTCGTGCAA
CGTTAGTAGGACAGTAGTAGTAAGCAAAAATACGTCGTCAATATATATGTGGTGCTTCTC
GTAAATATCTCCGCGGAGCAAACTTTTCTATAAAAATTAGAACCGACTCGCTGCCTAATC
CGTAACTAGTTCCCAACTTCGGATATATTAAGCAAGAATCCCACCCGTTAATCCACTTGC
GTGCATCCATAGTGCCCGCATAGCGTACAAACAGCCAACCGAGACATCCTGACTGCATCC
AGCCGGGCCAAGGTGCGCTAGTATTGTTGGTCGTAGGATTTACTTGCCAGCAGCAAACTC
GCTACCGAGCGAGAGCCCAGCGGTATGTTTCCTTTTCGACTCACCGCCAAAGGAAAAAGG
TTAATCTCTCGCTTGGTAATCGCGTCACAGGTAAAGGTAGTCACTCGCACGCTGTGTCCC
AGCTTGGCCGGATTGTAAGATGCTTCGTAAACCAGTTTACATGAGCTGCCTTCAGAATAT
CAATTAGCGTCCAAACCGGACAATGCAAGGGAATTAGCTCATAGCTCTTGGGCCTTTTTT
CTCAACTTCCTAATCGTAGACATCTTGCCTGGGATTTCAGTTTGAGCGCGTATTAAGCAG
CGAGACAGACTTCGGCTCTTATATATTACTAGACAGAAGCATAAGAGTCAAACAGTATTC
ACCTATTCAGCTACCTCTAGGTAGTTACTGGTCTACGTAAAACTTTATCATTACTGTACA
ACAATGTAGTACCGTTCCAGTATACCCAGAGCAGCGCATTGCAGACCACATACTACGCAT
TGTTATTGTTTACTTTGTGCCGATAAGCCGTATCCAATGTATATAGGACAAGTGGCCAGT
ATCGACAGGCGGATGTATTCACATCTAAAGCACATAACTGTAGCGTGAGGCTACCACGTT
ATATAGCGACTATAGCGTATTGGTAACTGAAAGAAATTGCGTATGTATAGTAGTTTGTAG
TGCGTTAAAACTAAAGTGGAATGCTCCTCGAGCACTGAGAGAAAGAGACTATGCTATTAC
ATGGTTGTTTAACTGACGATACTAGACGGTTCATTGCACAATAACTAGAATCTAAGAGTC
GTATACTAGTTAGCTGGCTATAGTATTATTGGGGTTTTGGATATGTCATTTTATTGTGTT
GATTAAGAGAGTACCATGCAGCATACATACATACGTTGTATTGTGTATTATATTGAGTGA
GTAGTATTAGAATGTCGGGTATATACTCAAAAGGCCTCCTATACCTCGAGCGTAACATAT
GGTGTGGCCATTGGAGCTTATTGCAACAGTTGTTCTGCGACGCAAGCGTTATAAAGTTGG
ATTATGACTTAGTAAAGACCGCAATAACATAGTGGTCGTATTGCCGATGGAATTGGGTCA
TATCGGCGATGTAACTTATGGGCGTTTGTCATAAGCCGATATCTCGTGGATTAGCAGAGT
ATCTTCGCCGATCGAGTAAGCAAACTGTTTTCTTTTGCAGAATTTCAGGTTATTTGACAT
CCGAAGTAGCTAATTGCGTTTTAGACATACGGTATCTTGAGAGCTTACTCTGTTGTAAGC
TAGCGGGTGTTATACACTTGTGGTCAATCTTCAGCACCTGTCTTTAATATG