Aniseed - Construct

Construct

ID

CONS00001246

Name

p.Cirobu.REG.KH2012.C9:5648367-5649375>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord.

This construct is testing Regulatory Region

REG00001243: Cirobu.REG.KH2012.C9:5648367-5649375|DDR1; DDR2; MUSK

Sequence

Show sequence

Length: 1,009

AATCTCACTTACTTGCGAAGGTGACATCACCCGGTGTGACGGATCTCAAACCAGGTTTAT
TTACTTCCTCGTGTGTAAATAAACAAATACAGATCGCAAGGCTTTGTCATATTGAACTCT
GAAAGCCACCGCTGTTGCGCCAATAACAGGGACCATAACCGGCACAACTACCAGTACAAA
GTTGCATGAAACAATATCGACAGTTTCAATACAAACAAATTATCATAGTTTTATGCAAAC
ATTTGTTCAAGCGACGTATTATCCAAACATAGAAAAAATACATTTCTTTTCAAAGAAGTC
ATTGTGAAAAAATCCTATGAAATGGTTGTATACCCACTTTTAAATTCGTCATACCAAGTT
CAAACAAAAGAACAAAAATAGCGATTACGTAATTTGAGTTGTAAGAAGGTGACCGATTCT
TGACACGTTCCGGGGCCGGGTCGTCTTTTTGCCGGGGAGCGTGCTCAAATTTTACGATCA
TTCTCGGAGTAGCTGAACGACGTCCGTGAGGTAATTATAAGGCGGGGTCAATTCCACGAA
AACGCACTGCTGGAGATACGGTGTTCAGAGACGCATTGTTCAACGAGGGTTTACGACAGG
CAGTTACAGTAAATTGCTGCTAATAATTCGTTAAATAGTGCCAAGTAATTTTAGAAGATC
TTCGGAATTAAAATGAAAATTATTTTCAAATGATTCGATTGATTTGATAGTTTGATAACG
CTAGAAGTTCGTCCCAATATGACCTTTCCACATTACGATGTCGGTCCTTCAAAAGTATGT
TGTGTTAACGGCGAGTGTACTACTTTCAATCGAGCAAATATTAGATACAAAAATGGGTCA
GTACTTCAAACTTAAGGTTTCGCGATTGCTGGCGCTCTCTGTTTGTGTAGTAGGGAATAA
ATTATAACCATTGGTGTTTGTAAACCGGTCAAATGTTGTCTTGGTTCAACAAAACCTGGA
CAGTTTGATTGGTGTTAATTTATGAGCAACAAAATGCGCTCCCATGTTT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data