Aniseed - Construct

Construct 'CONS00001243'

Construct

ID

CONS00001243

Name

p.Cirobu.REG.KH2012.L5:133066-133414_Bra3mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in notochord, mesenchyme and central nervous system.

This construct is testing Regulatory Region

REG00001240: Cirobu.REG.KH2012.L5:133066-133414_Bra3mut|Pavarotti-like

Sequence

Show sequence

Length: 349

CAGTGGACAAGTTTGTGAAGGAATCAATTCTATTTCTTTCGTCTGGAAAATAAAATATAA
TAAATGAATGTAACTTATTTATCCTCGCGTGGTGAGGCAACGACAGTCGTTATAACACTG
GTGTTCTGTTTCATACACCTCGTGCCCGCTTACAAGTTACCACGTATGTAACTTATTTAT
CCTCGCATGGGGAAGCAACGATAGTCGCTATAACACTGGTGTTCTGTTTCATACACCTTG
TGCTCGCTTACAAGTTACCACGCATGTAACTTGTTTATTCAACCATGGCGGGGCAACGAT
AGTCGTTATCAAGACGGTGCTCTGTTTCACACACCTCGTGCTCGCTTAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data