Aniseed - Construct

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Construct 'CONS00001258'

Construct

ID

CONS00001258

Name

p.Cirobu.REG.KH2012.C7:40900-40980>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the mesechyme and very weak expression in notochors and tail muscles.

This construct is testing Regulatory Region

REG00001255: Cirobu.REG.KH2012.C7:40900-40980|Noe2; Olm2A/B

Sequence

Show sequence

Length: 81

GAATGTTACGCGTTAGAAAGTCGAAACAGGGCGAACAAATACTCTTCGGTTCGGCACTGA
TGCGAAATTAGACAAACAGTC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data