Aniseed - Construct

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Construct 'CONS00001254'

Construct

ID

CONS00001254

Name

p.Cirobu.REG.KH2012.C9:5648367-5648505_Fox1/Bra4mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives weak LacZ expression in the notochord, mesenchyme and tail muscle.

This construct is testing Regulatory Region

REG00001251: Cirobu.REG.KH2012.C9:5648367-5648505_Fox1/Bra4mut|DDR1; DDR2; MUSK

Sequence

Show sequence

Length: 139

AATCTCACTTACTTGCGAAGGTGACATCACCCGGTGTGACGGATCTCAAACCAGGTTTGG
GCAGTTCCTCGTTAATAAATAAACAAATACAGATCGCAAGGCTTTGTCATATTGAACTCT
GAAAGCCACCGCTGTTGCG

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data