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  1. Construct 'CONS00001261'

Construct

ID

CONS00001261

Name

p.Cirobu.REG.KH2012.C7:40900-40993_HDmut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives weak LacZ expression in the notochord and in the central nervous system ; and strong expression in tail muscle and mesenchyme.

This construct is testing Regulatory Region

REG00001258: Cirobu.REG.KH2012.C7:40900-40993_HDmut|Noe2; Olm2A/B

Sequence

Show sequence

Length: 94

GAATGTTACGCGTTAGAAAGTCGAAACAGGGCGAACAAATACTCTTCGGTTCGGCACTGA
TGCGAACGACGACAAACAGTCTCGCTCTGACCGC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

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