Aniseed - Construct

Construct

ID

CONS00001296

Name

p.Cirobu.REG.KH2012.C7:3853138-3854780>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord.

This construct is testing Regulatory Region

REG00001277: Cirobu.REG.KH2012.C7:3853138-3854780|Ephrin3

Sequence

Show sequence

Length: 1,642

ACGGTTAGGTTAAAGGCCCACAGGAAACCTCTGGTTTGGCACCGGTTATTATCAGCGCGT
CTAATAACACAATGGAAGTGGTCAAGTCCAATTTAATGGCTCGTTTGAGCGTATACTTTT
GTGCTCATGACACTTCATAATAATCATGCAATGTTACACTGGCGCCACAAAAATTCTGTC
AGCATGTTTGACAAAAAGGATTCGTTTTGAACTGTCTACCTTAACATGCATAAAATCTCT
TTGATTACAATAAAGCTCATTAAAATAACAGCAAATAGACGATTTCGCAACAAAACTACA
GTTGTTATAACTAAACACGCTTGGGATAAATTAAATAAAAGAGCATTGGTTTAAAGCGGT
TCGTACGGCGAAATAACAGTCGTTATAACACGCTGTATAATAAGTATAATGAAAACAAAA
CAGTTAAAAAGCTTGAGTTACAGCAAAACCACAGTCGTTATAACGCGCTTATAGAATAAA
ACAAACGAAACTGTTAAAAAGCGTGGCTGTTAATCGAACACAGTCTATTCTTTGTCACGC
GATCAAAGACTATTCTTACGCAATATTAGGTGAAATCCACAGTCGTCCCGTTACTTTACA
AACGCCGCTTATAAGAGCCTTTGTCACGTTAGCAAACATGCCGCTGTAAAACATTTCGTG
TCAATATTTTCCGCTGTAGTTTGATCGCTTAAGCAATTATTGAATATGCCGTGTTAAAAG
CGGCCAATTCGGTATATTATAGCAAAGATAATACTGTTAACATTACGACATTGAAATCTA
AATGTATTGAACGGTAAACGTCGTATTGAAAAATGAATCTCTATTTATAACTCTCTACAG
TATACGGAGAGTCCCCACCCATTAAACAAACGTAATATTAATTGTTAGATAGATATGATT
CACACAGGATGTATTTGTGGTGTCTGGATTAACCCCCAGTAAGAATTCTCTGCCATACAA
TAGCAGCGTGTTTGAATTACTGCTTGTTTTGGAAGTTGATTAAGTCAGGTCAATCTAGGT
CACACACACACACACACTGTATGTATATACACAACACAGATGTAATATAAACATTGTAGT
ATTGAAATAGTAGTAAACCATTTTAAATGTACTTAGCTCTGAGCACATTGTATGTTGTAT
GCCATATATAGTAGGGTGGGGGAAGATGGGACACCTTTTCATTCTATTTTCTCGTTCCAG
TTGGTAGTAAACAAAGAACGTTCAAAGAATTATTGGATATTGTGTGCTTAAGGTTTCCCA
TCTTTCCCCATCCTACTATATATGCAGTACAGACGCTTGTTTTATCTTGATTGAAAAGTA
ATGCATTATGCATGTAGTAACAATAATGTACATTCTTTAAAAGTCCAAACATAATTAAAT
GATTTTATCTCCACACAGGCTGCATGGTTGGCAAGTACAAATCTACAAATGGCAATACAC
CTTGTCAAGTCTGCCCACAGCACAGCGTAACACATTCAACATCCGCTAGTCACTGTACTT
GTGTGGCTGGACATTATCGAGCCGAAAACGACCCAATCAGCCAAGCCTGCACTCGTAAGT
AGTTTTGTCAAATATTTCACATTCCTTTCGAAATATGATGCCGATGTTTCCTTTGGTTTT
TAAAACAATGCCTCTAATATTT

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data