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CONSTRUCT

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  1. Construct 'CONS00000784' > 
  2. Construct 'CONS00001277' > 
  3. EST 'AHC0AAA231YH03_RM1' > 
  4. Clone 'AHC0AAA231YM14' > 
  5. Construct 'CONS00001284'

Construct

ID

CONS00001284

Name

p.Moocci.REG.ELv1_2.S145165:64532-65350>NLS::LacZ

Min Promoter

Undefined Minimal Promoter

Reporter

LacZ

Comment

1.2kb Mesp regulatory sequence drives nuclei-localized LacZ expression.

This construct is testing Regulatory Region
Sequence

Show sequence

Length: 1,168

GTGTTTAGCGATCACGGTTCAAGTTTAGGCTACATCGCTTTTTCCATCGGTTTGCCTAAA
CTTGCATGCTGTATACAGTATTAAGAAGGTCTTGTTTAACAATGTTCCTTAAATGTTATT
GACATACAAAGCAAACTTTGTGTTAAAAGCCTACTTTAATGTGAAGACCAACATAAAAAA
ATTATCGGATAGTATAACTTACTCAAAAAAGTTAAGTCTTTGTCCTCTTTGCAAAGCTGC
AGTAACTTTATCGAATCCCCCAAGATACACGGATGTTCTGATGATAACTAGAAGCATCCC
CACTATCATTACTCCAGCTTGTACAACGTCTGTCCATATCACACCTTTTAAACCACCCTG
GAAATTAAATCATTATTATTATTAAAGATAAAAACCATTTATAAGCCTATTTACAATACA
GTTCCAAATTTAATCTGCCAATTCGATTGAACGTTTTTCAATGAAGTAAAATATCTCTCA
CAATCTTGTATTGAGCCTTTAAAATAGCCTTTTAAATGAAAATCTATATCTGACAAAATA
GGCTTTGACGTGATTTGAACGCCGTACCTCATGCTCGTATAAATACCTAATTTGTTGCGG
AATTTCTACTTCAATATAAAAAGTATGCAAATACATACATAATAGTTATTGAAAAGAATT
TAAATCATCAAATTTTATGTCTGTTGTAGCCTAAATAGTGTTTTATTTAAGTAAATCTAC
ACTGCGCTTCAGTTCAAACCAACCGTGCCTTGAACTACGGGCAGTGCGGTTGCTATAGAC
GTTATGCTTACGTAAACTAGTGCAAAGAATTTGTTGGAAAGTGTTAATTGATATCCGACC
ACCGACTGTGTTATCTTATCGTTTTGATAGATAAGAGCTGATAACACTGATAATAGACTA
ATTAACTATAGCAGTTAATTTTTATTAAAATTTTCACTTTTCGGACATGAAAATTTGTCT
AAAACCCAAACTTCCAAACTTCATCTATATAAGGAAAGAATTTTATTGAAATGGCATACT
CAATTCATCAGTATATATCATCTATATCTATATACGGTGGGTAATACGTCGTAATTGATA
TCGGACAAAGTAAAAACTGCATATTGAAGCGTTAGAAGAAAGTTGTTCAATTCAACATTT
TAACAAAATGGACCAAAGTGTACTGGGA

Reference(s)

Stolfi et al. (2014). Divergent mechanisms regulate conserved cardiopharyngeal development and gene expression in distantly related ascidians. Elife 3():e03728

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