Aniseed - Construct

Construct

ID

CONS00001307

Name

p.Cirobu.REG.KH2012.L13:46690-47600>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

Genomic fragments for enhancer discovery and analyses were cloned into the pFBΔSP6 plasmid, which contains the LacZ reporter gene. The tested enhancer drives LacZ expression in the notochord.

This construct is testing Regulatory Region

REG00001288: Cirobu.REG.KH2012.L13:46690-47600|KH.L13.2

Sequence

Show sequence

Length: 911

CAATTATCTTAACCTGACATTTGCCCTTGTGTGTTACCTCGTAGATCCCTGCATTATAAC
TAAGATACTGTCTTAACATCCATGATATTTCTAATCTAATTGATCAATTCCGTTTTTACA
TATATACGCGGGAAATGCGCGTGGTAAATTCCATATTACGCAGTCGTATATACGTCACCA
GTATTCCCATGATGTCACTAAAACGACGTTACGCCATATGTACGTGTGATACCGGTATGC
TCGGTACGCGACTGGCGGTTCTAATTTCGATACCTTTTATTCTGGCAAGCTTATTTCACG
TGAACTCTCTTAACAAAGGTCGATGCTATTGAAATCTTATTTTCAATTGACCAATGTGCG
TCATACACGACATACACCCGAACCTGGTATATGCTGGAATAATGAACATATTTAACTCAT
ATTCATTTGAAATAACGGCCTATTGAATTTAAAGCGATAATTGCCCTTTAAGAGCTCACG
TCCATAACGCTTATTGCCTCTCCATCTTTGGAACTGACTCTGTACATAAAGTGGCTTCAA
CTAAAACCTATTTAAAATCTATCACTTTACTGAGAAGAGTCAAGTGCGAACATTGGCCGG
GAAGTGGGTGACCGTTGAATCCGGCTTATCCACGCTGGCTGACTTACCAGATCGCTTATT
AACTTTTTAACTCCATTACGTCCGTTATCAAGCTGACAACTGGCGCGAATGGAATCGTAA
CTCTATATATATTATAACTCAATCTACCGAGACGACGGGTTATCGAAGAAAAACATTCTT
GTCTGGCACTCTCTCACCTAAGTGCCTTATAAGGAGTTAGTCTCTCGACTTAGTAAAAAG
TAACTAACGTCGCTGCGAAGAGTGATACTGGAGTAACAATGACTCATTAAGCCGCGATAA
CCAAATGCTAC

Reference(s)

José-Edwards et al. (2015). Brachyury, Foxa2 and the cis-Regulatory Origins of the Notochord. PLoS Genet. 11(12):e1005730

Show in situ data