Aniseed - Construct

Construct

ID

CONS00001313

Name

p.(Cirobu.REG.KH2012.S654:26922-27123_μA)x4>GFP

Min Promoter

pBra

Reporter

GFP

Comment

Four repeats of the critical upstream sequence of Tbx6.b with mutations were ligated to the Brachyury basal promoter, upstream of GFP.

This construct is testing Regulatory Region

REG00001293: Cirobu.REG.KH2012.S654:26922-27123_μA|Tbx6.b

Sequence

Show sequence

Length: 812

TTATCAAACCAATATATTGTGCATGGAACGGTATGCGTACACTGAGTTTTGGAGTGTTCT
GCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCT
TGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTT
TAGTTTCACAGTACCTCATTCCGTTATCAAACCAATATATTGTGCATGGAACGGTATGCG
TACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAG
TCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAA
CGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTACCTCATTCCGTTATCAAACCAATA
TATTGTGCATGGAACGGTATGCGTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACA
CGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGT
TAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATAAAATGGGTTTAGTTTCACAGTAC
CTCATTCCGTTATCAAACCAATATATTGTGCATGGAACGGTATGCGTACACTGAGTTTTG
GAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGC
AGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCGCTATA
AAATGGGTTTAGTTTCACAGTACCTCATTCCG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data