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CONSTRUCT

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  1. Transcript 'Harore.CG.MTP2014.S6...' > 
  2. Transcript 'Phfumi.CG.MTP2014.S9...' > 
  3. EST 'AHC0AAA229YC12_RM1' > 
  4. Clone 'AHC0AAA235YG23' > 
  5. Construct 'CONS00001318'

Construct

ID

CONS00001318

Name

p.(Cirobu.REG.KH2012.S654:26922-27123_μF)x4>GFP

Min Promoter

pBra

Reporter

GFP

Comment

Four repeats of the critical upstream sequence of Tbx6.b with mutations were ligated to the Brachyury basal promoter upstream of GFP.

This construct is testing Regulatory Region

REG00001298: Cirobu.REG.KH2012.S654:26922-27123_μF|Tbx6.b

Sequence

Show sequence

Length: 816

TTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCT
GCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCT
TGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCTATCGCGGGGCAGGT
TTAGTTTCACAGTACCTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGC
GTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGA
GTCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAA
ACGAGTCTCTATCGCGGGGCAGGTTTAGTTTCACAGTACCTCATTCCGTTATCAAACCAA
CGCGCCACATGCAAGACGGTATGCGTACACTGAGTTTTGGAGTGTTCTGCATGCGCTAGA
CACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGAAGCAGTTGTCCTTGTTAATGACTC
GTTAAGATCAAAGTGACATCGAAAACGAGTCTCTATCGCGGGGCAGGTTTAGTTTCACAG
TACCTCATTCCGTTATCAAACCAACGCGCCACATGCAAGACGGTATGCGTACACTGAGTT
TTGGAGTGTTCTGCATGCGCTAGACACGAATCAGCAGGAGAGTTCGGAGTCTTATCAGGA
AGCAGTTGTCCTTGTTAATGACTCGTTAAGATCAAAGTGACATCGAAAACGAGTCTCTAT
CGCGGGGCAGGTTTAGTTTCACAGTACCTCATTCCG

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data