Aniseed - Construct

Construct

ID

CONS00001086

Name

p.Cirobu.REG.KH2012.C8:2621713-2622953_μTcf7(x5)>GFP

Min Promoter

Endogenous Promoter

Reporter

GFP

Comment

Reporter gene construct in which mutated Foxd.b upstream regulatory sequence was fused to the green fluorescent protein (GFP) reporter gene.

This construct is testing Regulatory Region

REG00001117: Cirobu.REG.KH2012.C8:2621713-2622953_μTcf7(x5)|Foxd.b

Sequence

Show sequence

Length: 1,241

TTTGTTTACTTTTTTTACCCCATTTATGTTAAGTAAAGTTTAATTTCATTAAAGATGTTA
GTTCGACCCCAATCGTAGCTTAGCCCAACTGATGTCGCGAGCGATTAAGCGATAAGAAGG
CGAGAAATATCATCGATGCCCATTCCCGATATTTGGCGGCGTTTGTGGCGCCCAAAAGAG
GCGAGTTCGAGTCGCGAGTCTCGATCTTTTCATCTTTATCTCGGTTGAATTGATTTGTCG
TGAATAAATTGGGAAGTTGTGTCGATTGGAACATCAGTTGCAAGAATGAGTTGAACAGTT
GGTCCAATTCAAACGTTACTTGCAGTAAAACTTGTGTGGACAAGTTATAACTTAATACGA
GGTGTATATAAAGTCGTTAGAATTTTACGAATCAGTTGACAAATATAAATAAAGGTACAG
GTAGTTTTGTTGAACATTGCGACAGTCTGACTGTGTTTTCAGTTTCGCTAAATTTCTCGT
ACGGTAAAATTGTTTACTAAAAAATAAGGGCGTACGTAAATCAATTCTGGGTTGGGTAGC
TTTAAGCATTACAATAACGTACATATTGCCTGTTTACAAATATAGCAGTTATATTTAAAT
ATATACTAAGAATAAAGGTGCAGCTCATTCAAATTTAATCGCTTTACCGATTCTCTCAAT
TAAGAATAGGAGAAAACGCGTATTTAGATAACATTTAACGGGAAACGACTACAGTCCTAA
TATTGGAACTACAGACGTTCAATAAACTCGTTAGCTTGACATTTATTTCAATACTGTTTG
TGTTACGTCACGGGTATTTTTGAGTCATTATTTTAACGATTGGTCGGATTATTTGAAAAC
CTTTACCACAGTGTGTTGTTAGAAAATAGACGCCATTTTTACCAGCGGAACCAGCATTTT
GCCAAATTCAAATTTAATAATTTTTTTCAGTTGTTTTCAACATTTTGGGACACTTCTCAA
AAAAGCGAAATGTTTTTTATGAAACTCAGTTTTTAAAAACAGCGAAAACACGCATATTTT
TGAACGCAACCATTAATCGATTAAGTTGGACGTTTTTCTTGTAAATGATAAATTAATTAG
CCTTCTTCTTTTGCTTAGAAAACATTGTAATATAATCTTTTGAAAAATCTGTTGCTGATC
AACGGAATTATAAACGAGATCTAGGATTCGAAGAAAAAAGCGGTTGCTGGGAGAATTGAA
GAAGTGGATTTGAATAAATTGTCGCGTTTTGCAAAGTGCTC

Reference(s)

Oda-Ishii et al. (2016). A Maternal System Initiating the Zygotic Developmental Program through Combinatorial Repression in the Ascidian Embryo. PLoS Genet. 12(5):e1006045

Show in situ data