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- Transcript 'KH2012:KH.C4.69.v1.A...' >
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- Construct 'CONS00001639'
Construct
ID
CONS00001639
Name
p.Cirobu.REG.KH2012.L59:1807-2488_GATAmut>LacZ
Min Promoter
Endogenous Promoter
Reporter
Comment
This GATA sites mutated cis-regulatory element drives LacZ expression. Compared to the WT construct, the mutation of the 2 proximal GATA binding sites greatly reduced its activity in both NN (notochord and nerve chord, A6.2 and A6.4) and MM lineages (Muscle, mesenchyme and notochord, B6.2 and B6.4).
REG00001555: Cirobu.REG.KH2012.L59:1807-2488_GATAmut|Zic-r.b
Show sequence
Length: 681
TAGTTTATGTCATTTCCGTTCACTAAATGATATTTAAATTATAATCCTCTATAATCTTCA
CACACGCTACAATGGCTCGAGACAACCCGAATTTTAAACAATGATGACGTAACAATTAAC
AAAGGGTCTGGAATGTTGAATTCGCAATGACTTGCTGTTATCTAGGTTGTTATAAGATTA
TTTTATAATTTTAAAAAGTTTAAGAACATGCATATAAAAAAAATCTGATAAACTATAATT
TTAACACAATCGGTATGCTAATATTAGTAGTTAAATCAAACATAAAAAAATAAGCCGGCA
AATACATGCAATATTTTTTATTAAACAAAATAATTAAAAATCGAATACGTAAAAATTATT
AACAATTATAATTTATTATATATTTTTTTTAAATATCGATGAACGTCCTGTTTTTTGGTC
TTTTTTGCATAAACCACTCTGCAATGTGTAACTTGTTGTTGACTTCACGTAGAGAAAGTG
CCATAGGTAAACCTTGCGTAGACAGGCACGAGCGAAAGCGTGCCAAAAGTACCGCGAGAC
GAGCGATCGACACGATAATTACCTTCGCGATGTAAATTTCGGAGGTGGGGGGTCGAACCA
CGTGCGCATGGGAAAGCGACGGGCGATCGGAAGTCAAATAAATAAAGcgAAAGAAGcgAA
TTAAGTCATTGTGTTACGAAA