Aniseed - Construct

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Construct 'CONS00000545' >
Construct 'CONS00001669'

Construct

ID

CONS00001669

Name

p.Cirobu.REG.KhL96:471236-471527_Tbox5-mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

The 294bp notochord enhancer of Tbx2/4, carrying a mutation of its 5th T-box binding site, was cloned upstream of the basal promoter of Forkhead and LacZ reporter gene. This construct is active in notochord mainly and in mesenchyme.

This construct is testing Regulatory Region

REG00001579: Cirobu.REG.KhL96:471236-471527_Tbox5-mut|Tbx2/3

Sequence

Show sequence

Length: 292

GTGGACAGACAGGGTAGTTAAATACGTTACAAAGTTCATTTGCCACCCAGCACAGTATTG
TACTTTATGACACTTGTGTTTATTCGTATAGTGGGACTAGTTATAAAATAAAACGGTTTA
TTCTCTGACACGTAAataTTAATCTATAAGCAATGTGACAAGAACAAATAACACGAAGGG
GCGAAATCTAACGCTAGATCAACTGCGTGGCTTTCACATCTTTGGTGGTCGAGTAATACA
TAGCACTAAACCGAAACAATTATTTTTCGTCGACTACCGTAATCCCCATTCC

Reference(s)

Show in situ data