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CONSTRUCT

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  1. Construct 'CONS00001285'
  2. Construct 'CONS00000023'
  3. Construct 'CONS00001276'
  4. Construct 'CONS00001671'

Construct

ID

CONS00001671

Name

p.Cirobu.REG.KhL96:471236-471527_Tbox7-mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

The 294bp notochord enhancer of Tbx2/4, carrying a mutation of its 7th T-box binding site, was cloned upstream of the basal promoter of Forkhead and LacZ reporter gene. This construct is active in notochord, head epidermis and tail muscles.

This construct is testing Regulatory Region

REG00001581: Cirobu.REG.KhL96:471236-471527_Tbox7-mut|Tbx2/3

Sequence

Show sequence

Length: 291

GTGGACAGACAGGGTAGTTAAATACGTTACAAAGTTCATTTGCCACCCAGCACAGTATTG
TACTTTATGACACTTGTGTTTATTCGTATAGTGGGACTAGTTATAAAATAAAACGGTTTA
TTCTCTGACACGTAAGTGTTAATCTATAAGCAATGTGACAAGAACAAATAAtatAAGGGG
CGAAATCTAACGCTAGATCAACTGCGTGGCTTTCACATCTTTGGTGGTCGAGTAATACAT
AGCACTAAACCGAAACAATTATTTTTCGTCGACTACCGTAATCCCCATTCC

Reference(s)