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  1. Construct 'CONS00001338'
  2. Clone 'AHC0AAA222YK24'
  3. Construct 'CONS00001394'
  4. Construct 'CONS00001319'
  5. Construct 'CONS00001674'

Construct

ID

CONS00001674

Name

p.Cirobu.REG.KhL96:471236-471527_Tbox5-7-mut>LacZ

Min Promoter

pfkh

Reporter

LacZ

Comment

The 294bp notochord enhancer of Tbx2/4, carrying a mutation of its 5th and 7th T-box binding sites, was cloned upstream of the basal promoter of Forkhead and LacZ reporter gene. This construct is very weakly active in notochord and tail muscles.

This construct is testing Regulatory Region

REG00001584: Cirobu.REG.KhL96:471236-471527_Tbox5-7-mut|Tbx2/3

Sequence

Show sequence

Length: 291

GTGGACAGACAGGGTAGTTAAATACGTTACAAAGTTCATTTGCCACCCAGCACAGTATTG
TACTTTATGACACTTGTGTTTATTCGTATAGTGGGACTAGTTATAAAATAAAACGGTTTA
TTCTCTGACACGTAAataTTAATCTATAAGCAATGTGACAAGAACAAATAAtatAAGGGG
CGAAATCTAACGCTAGATCAACTGCGTGGCTTTCACATCTTTGGTGGTCGAGTAATACAT
AGCACTAAACCGAAACAATTATTTTTCGTCGACTACCGTAATCCCCATTCC

Reference(s)





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