Vegetal view of a Ciona robusta 16-cell stage embryo probed for Fgf9/16/20 expression and injected with β-catenin MO.
Fgf9/16/20 expression is lost.
| Stained molecule | KH2012:KH.C2.125 (FGF16; FGF20; FGF9) |
| Stained region(s) | No expression - |
Vegetal view of a Ciona robusta 16-cell stage embryo probed for Foxd expression and injected with β-catenin MO.
Foxd expression is lost.
| Stained molecule | KH2012:KH.C8.396 (FOXD1; FOXD3; FOXD4) |
| Stained region(s) | No expression - |
Vegetal view of a Ciona robusta 16-cell stage embryo probed for Foxa.a expression and injected with β-catenin MO.
Foxa.a is expressed in A5.1, A5.2, a5.4 and a5.3 blastomers.
Original comment: In β-catenin–inhibited embryos, Foxa.a expression persisted in NNE (neuro-mesendodermal cells) and a-lineage cells.
| Stained molecule | KH2012:KH.C11.313 (FOXA1; FOXA2; FOXA3) |
| Stained region(s) | A5.1 cell pair - A5.2 cell pair - a5.3 cell pair - a5.4 cell pair - a line - A line - |
| Stained molecule | KH2012:KH.C3.716 (EFNA1; EFNA4; EFNA5) |
| Stained region(s) | whole embryo - |
Animal view of a Ciona robusta 32-cell stage embryo probed for Zic-r.b expression and injected with morpholinos against beta-catenin.
Zic-r.b is only expressed in B6.4 blastomer pair.
| Stained molecule | KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | B6.4 cell pair - |
Animal view of a Ciona robusta 32-cell stage embryo probed for Zic-r.b expression and injected with Foxd mRNA and morpholinos against beta-catenin.
Zic-r.b is expressed in A6.2, A6.4, B6.2 and B6.4 blastomer pairs, and ectopically in the a lineage and A6.1.
Original comment: Injection of Foxd mRNA was able to rescue expression of NN(neural-notochord)-lineage gene Zic-r.b in b-catenin-MO embryos.
The green dashed line separates a- from A- lineages.
| Stained molecule | KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | A6.1 cell pair - A6.2 cell pair - A6.4 cell pair - a line - B6.2 cell pair - B6.4 cell pair - |
Animal view of a Ciona robusta 32-cell stage embryo probed for Zic-r.b expression, injected with Foxd mRNA and morpholinos against beta-catenin, and treated with U0126 from the 16-cell stage
Zic-r.b is expressed in B6.4 blastomer pair, and in some cells from the a-lineage or the NN lineage (neural and notochord.
| Stained molecule | KH2012:KH.S816.1 (ZIC2; ZIC3; ZIC4) |
| Stained region(s) | A6.2 - A6.4 cell pair - a6.5 - a6.7 - B6.4 cell pair - |
Vegetal view of a Ciona robusta 64-cell stage embryo probed for Brachyury expression and injected with morpholinos against beta-catenin.
Brachyury expression is lost.
| Stained molecule | KH2012:KH.S1404.1 (TBX19; TBX6; TBXT) |
| Stained region(s) | No expression - |
Vegetal view of a Ciona robusta 64-cell stage embryo probed for Brachyury expression and injected with Foxd mRNA and morpholinos against beta-catenin.
Brachyury is expressed throughout the A-lineage.
Original comment: Injection of Foxd mRNA was able to rescue expression of NN-lineage gene (Neural Notochord) in b-catenin-MO embryos.
| Stained molecule | KH2012:KH.S1404.1 (TBX19; TBX6; TBXT) |
| Stained region(s) | A7.3 cell pair - A7.7 cell pair - A line - |
Vegetal view of a Ciona robusta 64-cell stage embryo probed for Brachyury expression, injected with Foxd mRNA and morpholinos against beta-catenin, and treated with U0126 from the 16-cell stage.
Brachyury expression is lost.
Original comment: Injection of Foxd mRNA is not able to rescue Bra expression in b-catenin-MO embryos when FGF-signalling pathway is inhibited.
| Stained molecule | KH2012:KH.S1404.1 (TBX19; TBX6; TBXT) |
| Stained region(s) | No expression - |
