RNA-seq experiment
Author
Lemaire team (2017-07-01)
Annotator
Lemaire team (2017-07-01)
GEO Accession Number
SRA Accession Number
Oocytes of a single individual of Halocynthia roretzi (Aomori, Japan) were fertilized by sperms of an another single individual and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Total RNA was prepared with the AGPC method and the quality was checked with the Bioanalyzer, most samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China).
| Biomaterial | Number of reads | Library name | |
|---|---|---|---|
| Stage 0 (Unfertilized egg) | whole embryo - | 46205098 | Harore_RNA-Seq_stage1_WT_Replicate1 |
| Stage 8 (64-cell) | whole embryo - | 52830074 | Harore_RNA-Seq_stage8_WT_Replicate1 |
| Stage 12 (mid gastrula) | whole embryo - | 60527173 | Harore_RNA-Seq_stage12_WT_Replicate1 |
| Stage 21 (mid tailbud I) | whole embryo - | 51622970 | Harore_RNA-Seq_stage21_WT_Replicate1 |
| Stage 26 (hatching larva) | whole embryo - | 61671158 | Harore_RNA-Seq_stage26_WT_Replicate1 |
| Biomaterial | Number of reads | Library name | |
|---|---|---|---|
| Stage 0 (Unfertilized egg) | whole embryo - | 59011922 | Harore_RNA-Seq_stage1_WT_Replicate2 |
| Stage 8 (64-cell) | whole embryo - | 48292546 | Harore_RNA-Seq_stage8_WT_Replicate2 |
| Stage 12 (mid gastrula) | whole embryo - | 52979014 | Harore_RNA-Seq_stage12_WT_Replicate2 |
| Stage 15 (mid neurula) | whole embryo - | 55311936 | Harore_RNA-Seq_stage15_WT_Replicate2 |
| Stage 21 (mid tailbud I) | whole embryo - | 54631071 | Harore_RNA-Seq_stage21_WT_Replicate2 |
| Stage 26 (hatching larva) | whole embryo - | 61493911 | Harore_RNA-Seq_stage26_WT_Replicate2 |
