Aniseed - Gene Card

EST count

27 results

Library Name	Percentage	Expression
Ciona intestinalis whole animal Ciona intestinalis whole animal		0 clone(s) / 0
Nori Satoh unpublished cDNA library Nori Satoh unpublished cDNA library		0 clone(s) / 5,577
Nori Satoh unpublished cDNA library, young adult Nori Satoh unpublished cDNA library, young adult		0 clone(s) / 31,366
Nori Satoh unpublished cDNA library, cleavage stage embryo Nori Satoh unpublished cDNA library, cleavage stage embryo		0 clone(s) / 15,579
Nori Satoh unpublished cDNA library, egg Nori Satoh unpublished cDNA library, egg		0 clone(s) / 31,100
Nori Satoh unpublished cDNA library, larva Nori Satoh unpublished cDNA library, larva		0 clone(s) / 26,704
Nori Satoh unpublished cDNA library, tailbud embryo Nori Satoh unpublished cDNA library, tailbud embryo		4 clone(s) / 26,680
directional larval cDNA library directional larval cDNA library		0 clone(s) / 39
Ascidian hemocytes cDNA library Ascidian hemocytes cDNA library		0 clone(s) / 0
K. Inaba unpublished cDNA library, testis K. Inaba unpublished cDNA library, testis		0 clone(s) / 5,468
Stratagene UniZAP whole-larva library Stratagene UniZAP whole-larva library		0 clone(s) / 0
Ciona intestinalis larva Ciona intestinalis larva		0 clone(s) / 0
Nori Satoh unpublished cDNA library, blood cells Nori Satoh unpublished cDNA library, blood cells		0 clone(s) / 29,579
Nori Satoh unpublished cDNA library, endostyle Nori Satoh unpublished cDNA library, endostyle		0 clone(s) / 2,556
Nori Satoh unpublished cDNA library, cleaving embryo Nori Satoh unpublished cDNA library, cleaving embryo		0 clone(s) / 16,939
Nori Satoh unpublished cDNA library, gastrula and neurula Nori Satoh unpublished cDNA library, gastrula and neurula		0 clone(s) / 25,258
Nori Satoh unpublished cDNA library, gonad Nori Satoh unpublished cDNA library, gonad		0 clone(s) / 16,936
Nori Satoh unpublished cDNA library, neural complex Nori Satoh unpublished cDNA library, neural complex		0 clone(s) / 10,463
Nori Satoh unpublished cDNA library, heart Nori Satoh unpublished cDNA library, heart		0 clone(s) / 13,243
Yutaka Satou unpublished cDNA library, adult digestive gland Yutaka Satou unpublished cDNA library, adult digestive gland		0 clone(s) / 17,765
Yutaka Satou unpublished cDNA library, embryo whole animal Yutaka Satou unpublished cDNA library, embryo whole animal		0 clone(s) / 17,872
Yutaka Satou unpublished cDNA library, mature adult whole animal Yutaka Satou unpublished cDNA library, mature adult whole animal		0 clone(s) / 107,314
Nori Satoh unpublished cDNA library, juvenile whole animal Nori Satoh unpublished cDNA library, juvenile whole animal		0 clone(s) / 24,372
Nori Satoh unpublished cDNA library, mature adult whole animal Nori Satoh unpublished cDNA library, mature adult whole animal		0 clone(s) / 17,126
Ciona intestinalis whole animal stage3 juvenile Ciona intestinalis whole animal stage3 juvenile		0 clone(s) / 3,798
Yutaka Satou unpublished library (cicx) Yutaka Satou unpublished library (cicx)		0 clone(s) / 2,031
Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula) Gateway compatible cien cDNA library, Ciona intestinalis mixed embryonic stages (Egg to Neurula)		2 clone(s) / 188,431

RNA-Seq transcriptome profiles (by Experiment)

3 results

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

FPKM & RPKM

Click here to see the RNA-Seq Pipeline Protocol.
You can show/hide lines in the charts by clicking on it in the legend part.
You can see more details by clicking on a point and then, on the link which will appear.

Be careful, FPKM & RPKM does not permit you to compare a gene across different conditions or experiments.

Download FPKM & RPKM data

OVERVIEW

Expression profile summary from all experiments in WT and mutant conditions:

Relative Log Expression RLE

Be careful, RLE and 2^RLE does not permit you to compare a gene across different experiments.

Download RLE Relative Log Expression data

RNA-Seq Experiment n°34135

Oocytes of a single individual of Ciona intestinalis (Roscoff, France) were fertilized by a mixture of sperm of two individuals and development was followed microscopically. Embryos were snapfrozen in liquid nitrogen at the appropriate stage and kept at -80°C. Samples at stage 0, 8, 12, 15, 21, 26 were collected from the same fertilzation, while sample from stage 11 was independently collected from a different individual. Total RNA was prepared with the RNA II kit of Magerey Nagel and the quality was checked with the Bioanalyzer, all samples had a RIN value of higher than 9. Strand specific libraries were constructed and the sequencing (50PE) was performed with Illumina HiSeq 2000 technology (BGI, Hong Kong, China). Reads were aligned onto C.robusta genome and considered to reflect the expression of the C.robusta orthologous genes.

Assay platform	Illumina HiSeq 2000
Layout	Paired-end
Stranded	Yes
Read length	49
Normalized data types	FPKM RLE
Wild type - Replica 1
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

Wild type - Replica 2
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 0 (Unfertilized egg)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 11 (early gastrula)
		C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)
		C. robusta formely Ciona int. type A, Stage 15 (mid neurula)
		C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)
		C. robusta formely Ciona int. type A, Stage 26 (hatching larva)

RNA-Seq Experiment n°34138

Ciona robusta adults were obtained from the National Bio-Resource Project for Ciona (NBRP, Japan). 50 unperturbed and Foxd-morphant embryos were collected at the 32-, 64-, and 112-cell stages. RNA was extracted using a Dynabeads mRNA DIRECT Purification Kit (Thermo Fischer Scientific) and libraries were made with an Ion Total RNA-Seq kit ver 2 (Thermo Fischer Scientific). The libraries were sequenced with an Ion PGM instrument (Thermo Fischer Scientific).

Assay platform	Ion Torrent PGM
Layout	Single-end
Stranded	Yes
Read length	20-365
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Mutant
	*Deregulated molecule(s)*	KH2012:KH.C8.890 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 6a (early 32-cell)
		C. robusta formely Ciona int. type A, Stage 8 (64-cell)
		C. robusta formely Ciona int. type A, Stage 10 (112-cell)

RNA-Seq Experiment n°34139

The purpose of this project is to identify genes positively and negatively regulated by signaling molecules that belongs to the TGFbeta superfamily.

Assay platform	Illumina HiSeq 2500
Layout	Single-end
Stranded	Yes
Read length	101
Normalized data types	RPKM
Wild type
	*Territories*	whole embryo -

	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 1
	*Deregulated molecule(s)*	KH2012:KH.C4.125 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Mutant 2
	*Deregulated molecule(s)*	KH2012:KH.C2.573 -
	*Territories*	whole embryo -
	*Biomaterial(s)*	C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

In Situ Experiment Data

49 results

Click here to visualize with MorphoNet

Experiment data ( results)

C. robusta formely Ciona int. type A, Stage 1 (One cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : No signal was detected.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 4 (8-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : No distinct zygotic signal was detected.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 5a (early 16-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : No distinct zygotic signal was detected.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 6b (late 32-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : No distinct zygotic signal was detected.

Aniseed annotation :
No picture available for this stage.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

No picture available in the article.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A7.6 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Vegetal view of a 64-cell stage embryo injected with ephrin-Ad MO.

Original annotation:
MyTF displays an almost complete loss of expression.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Vegetal view of a 64-cell stage embryo injected with ephrin-Ad MO and treated with the MEK inhibitor U0126 at the 32-cell stage and probed for MyTF.

Original annotation :
MyTF is expressed in both the A7.6 blastomere and the anterior endoderm.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A7.6 cell pair -

C. robusta formely Ciona int. type A, Stage 8 (64-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : A7.6

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A7.6 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MYTF expression following egg micro-injection of a FoxD-a/b MO.

Original annotation : MYTF was down-regulated in TLCs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MYTF expression following egg micro-injection of a Snail MO.

Original annotation : MYFT was ectopically expressed in lateral part of B7.7 mesenchyme.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair - B7.7 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MYTF expression following egg micro-injection of a Neurogenin MO.

Original annotation : MYTF was down-regulated in TLCs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MYTF expression following egg micro-injection of a FoxD-a/b MO.

Original annotation : MYTF was down-regulated in TLCs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage wild type embryo probed for MYTF expression.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : A7.6

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo showing expression of MyTF in A7.6 cell pair.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo showing expression of MyTF. Embryos were treated with U0126 at the late 32-cell stage.

Original annotation:
A7.6 group genes are ectopically expressed in the anterior endoderm blastomeres upon MEK inhibition, whereas A7.6 expression is unaffected.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A7.1 cell pair - A7.2 cell pair - A7.5 cell pair - A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal views of 110-cell embryos injected at the 1-cell stage with torso-FGFR mRNA and probed for MyTF.

Original annotation:
In situ hybridization assays revealed consistent reductions in MyTF expression in A7.6.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MyTF after injection of FoxD::ephrinAd. This construct was used to drive Ci-ephrin-Ad throughout the vegetal hemisphere at the 16-cell stage (thanks to FoxD enhancer).

Original annotation: MyTF expression is observed in A7.6 blastomeres and in anterior endoderm.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A7.2 cell pair - A8.11 cell pair - A8.12 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo probed for MyTF following injection of FoxD::Nodal. This construct was used to drive Nodal throughout the vegetal hemisphere (thanks to FoxD enhancer).

Original Annotation: MyTF was sometimes ectopically expressed in the B7.7 lineage, indicating by arrow.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A8.11 cell pair - A8.12 cell pair - B7.7 cell pair -

C. robusta formely Ciona int. type A, Stage 10 (112-cell)

Vegetal view of a 110-cell stage embryo showing expression of MyTF. Embryos were treated with U0126 at the 16-cell stage (picture and graph) and at the early 32-cell stage, late 32-cell stage and 64-cell stage (graph).

Original annotation:
These embryos often displayed normal expression in A7.6 cell pair but additional patterns were also detected, including expression in A7.6 along with ectopic expression in the endoderm, loss of A7.6 expression along with gain of expression in the endoderm, or complete loss of expression.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetal view of a mid gastrula WT embryo probed for Myt1L (red) and Tyrp1A (green) expression and electroporated with Dmrt>LacZ (white).
Original annotation : Myt1L is specifically expressed in the medial a9.33 and a9.37 cells of row III during gastrulation.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	a9.33 cell pair - a9.37 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Vegetal view of a mid gastrula WT embryo probed for Myt1L (red) and Tyrp1A (green) expression and electroporated with Dmrt>LacZ (white). Second picture shows ISH channel alone.
Original annotation : Myt1L is specifically expressed in the medial a9.33 and a9.37 cells of row III during gastrulation

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	a9.33 cell pair - a9.37 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Myt1L is specifically expressed in the medial a9.33 and a9.37 cells of row III during gastrulation.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	a9.33 cell pair - a9.37 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Late gastrula stage wild type embryo probed for MYTF expression. No picture available in the article.

Original Annotation :
MYTF was expressed in A9.13, A9.15 and A9.29 cell pairs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair -

C. robusta formely Ciona int. type A, Stage 12 (mid gastrula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : TLCs.

Aniseed annotation : A staining is additionally detected in the a9.33 and a9.37 cell pairs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	a9.33 cell pair - a9.37 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a 110-cell stage wild type embryo probed for MYTF expression.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair - a9.33 cell pair - a9.34 cell pair - a9.37 cell pair - a9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a FoxD-a/b MO.

Original Annotation The expression of MYTF is down-regulated in a-line neural cells and A-line nerve cord cells.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a FGF9/16/20 MO.

Original annotation : MYTF was down-regulated in a-line neural cells.

Aniseed annotation : it seems that expression was also down-regulated in TLCs and in A-line nerve cord cells.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a Dll-B MO.

Original annotation : MYTF was down-regulated in a-line neural cells.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a Nodal MO.

Original annotation : MYTF was ectopically expressed in b-line neural cells. Staining in A-line nerve cord cells and a-line neural cells was not affected.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair - a9.33 cell pair - a9.34 cell pair - a9.37 cell pair - a9.38 cell pair - b line neural plate -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a SoxC MO.

Original annotation : MYTF was down-regulated in a-line neural cells.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a ZicL MO.

Original annotation : MYTF was down-regulated in A-line nerve cord cells.

Aniseed annotation : expression is not very clear in a-line neural plate.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	a9.33 cell pair - a9.34 cell pair - a9.37 cell pair - a9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a FoxA-a MO.

Original annotation : MYTF was down-regulated in a-line neural cells and A-line nerve cord cells.

Aniseed annotation : it seems that MYTF was also down regulated in TLCs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	No expression -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Vegetal view of a late gastrula stage embryo probed for MYTF expression following egg micro-injection of a Otx MO.

Original annotation : MYTF was down-regulated in a-line neural cells.

Aniseed annotation : it seems that MYTF was also down-regulated in TLCs.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : nervous system, mesenchyme.
Comment : Mesenchyme: TLCs, Nervous system: A9.13, A9.15, A9.29, a9.33, a9.34, a9.37, a9.38

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A9.13 cell pair - A9.15 cell pair - A9.29 cell pair - a9.33 cell pair - a9.34 cell pair - a9.37 cell pair - a9.38 cell pair -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

At the late gastrula stage, MYTF is expressed in row III of neural plate.
Original annotation : The expression of MYTF in control embryos at the late gastrula stage (shown in animal view). The expression is illustrated schematically in the lower right by red (palps) and blue (brain).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	row III neural plate -

C. robusta formely Ciona int. type A, Stage 13 (late gastrula)

Original annotation : The expression of MYTF in BZ1/2 morphant embryos at the late gastrula stage (shown in animal view). The expression is illustrated schematically in the lower right by red (palps) and blue (brain).
MYTF, which is normally expressed in the posterior row, is not affected in BZ1/2 morphants.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	row III neural plate -

C. robusta formely Ciona int. type A, Stage 15 (mid neurula)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : nervous system, mesenchyme
Comment : TLCs.

Aniseed annotation :
The dorsal tail epidermis staining probably corresponds to precursors of epidermal sensory neurones.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	A line neural plate - row III neural plate - row IV neural plate -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Original annotation :
Gene name :myelin transcription factor (MyTF; Zinc finger C2HC).
Expression pattern : nervous system, nerve cord, mesenchyme
Comment : TLCs; a variety of neuronal cells including the brain and epidermal neurons.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - caudal epidermal neurone (CEN) precursors - trunk lateral cells (A7.6 line) - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 19 (early tailbud I)

Early tailbud wild type embryo probed for MYTF expression. No picture available in the article.

Original Annotation :
MYTF was expressed in b10.76 cell pair (dorsal nerve cord).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	dorsal TNC ependymal precursors (capstone cells) -

C. robusta formely Ciona int. type A, Stage 20 (early tailbud II)

Lateral view of an early tailbud stage embryo stained with MyT1. Arrowheads in early tailbud embryos indicate weak but detectable expression.
Original annotation: Expression was observed in epidermal sensory neurones.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	caudal epidermal neurone (CEN) precursors - posterior sensory vesicle - trunk lateral cells (A7.6 line) - ventral anterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral and ventral views of a WT Ciona robusta tailbud embryo probed for Myt1 expression.
Original annotation : Myt1 is normally expressed in the nervous system (white arrowheads).
Scale bar: 100 μm

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - peripheral nervous system - posterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Lateral and ventral views of a Snail morphant Ciona robusta 32-cell staged tailbud embryo probed for Myt1 expression.
Original annotation : Myt1, which is expressed in the nervous system (white arrowheads) of unperturbed normal tailbud embryos, was expressed ectopically in the muscle cells (black arrowheads) of Snail morphants (n=24, 100%).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - peripheral nervous system - posterior sensory vesicle - primary muscle lineage -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Conversion to Aniseed format of data from Yutaka Satou's gene expression profiles of transcription factors and signalling molecule ISH screen (Development. 2004 131:4047-58).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - apical trunk epidermal neurone precursors (ATEN) - caudal epidermal neurone (CEN) precursors - palp neurone precursors - posterior sensory vesicle - trunk lateral cells (A7.6 line) - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of a mid tailbud wild type embryo probed for MYTF expression.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
MYTF was expressed in A13.473 and A12.239 cell pairs in the visceral ganglion.

Aniseed Annotation :
It seems that there is also expression in sensory vesicle.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of a mid tailbud stage embryo probed for MYTF expression, following the injection of neurogenin MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
MYTF expression was lost in the visceral ganglion (white arrow).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle -

C. robusta formely Ciona int. type A, Stage 21 (mid tailbud I)

Dorsal view of a mid tailbud stage embryo probed for MYTF expression, following the injection of Emc MO.
Red and yellow arrowheads indicate boundaries between the neck and the visceral ganglion, and between the visceral ganglion and the caudal nerve cord, respectively.

Original Annotation :
Ectopic expression was observed in the neck (black arrow).

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - neck - posterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 22 (mid tailbud II)

Lateral view of a mid tailbud stage embryo stained with MyT1.
Original annotation: Expression was observed in epidermal sensory neurones.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	peripheral nervous system - posterior sensory vesicle - ventral anterior sensory vesicle - visceral ganglion -

C. robusta formely Ciona int. type A, Stage 24 (late tailbud II)

Lateral view of a late tailbud stage embryo stained with MyT1. White lines demarcate boundaries between different focal planes.
Original annotation: Expression was observed in epidermal sensory neurones.

Stained molecule	KH2012:KH.C1.274 (MYT1; MYT1L)
Stained region(s)	anterior sensory vesicle - apical trunk epidermal neurones (ATEN) - caudal epidermal neurones (CEN) - posterior sensory vesicle - rostral trunk epidermal neurones (RTEN) - visceral ganglion -